Recombinant Mouse Cathepsin G (Ctsg)

Code CSB-YP006190MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP006190MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006190MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP006190MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ctsg
Uniprot No.
Alternative Names
Ctsg; Cathepsin G; EC 3.4.21.20; Vimentin-specific protease; VSP
Species
Mus musculus (Mouse)
Expression Region
21-261
Target Protein Sequence
IIGGREARPH SYPYMAFLLI QSPEGLSACG GFLVREDFVL TAAHCLGSSI NVTLGAHNIQ MRERTQQLIT VLRAIRHPDY NPQNIRNDIM LLQLRRRARR SGSVKPVALP QASKKLQPGD LCTVAGWGRV SQSRGTNVLQ EVQLRVQMDQ MCANRFQFYN SQTQICVGNP RERKSAFRGD SGGPLVCSNV AQGIVSYGSN NGNPPAVFTK IQSFMPWIKR TMRRFAPRYQ RPANSLSQAQ T
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Cleaves vimentin. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa.
Gene References into Functions
  1. these findings present evidence of an arterial-specific recruitment pattern centered on CatG-instructed adhesion strengthening. The inhibition of this process could provide a novel strategy for treatment of arterial inflammation with limited side effects. PMID: 27660294
  2. Cathepsin G activity may impair efferocytosis, which could lead to an accumulation of lesion-associated apoptotic cells and the accelerated progression of early atherosclerotic lesions to more complex lesions in Apoe(-/-) mice. PMID: 23934850
  3. SerpinB1 is critical for maintaining polymorphonuclear neutrophils survival by antagonizing intracellular cathepsin G activity. PMID: 23532733
  4. Cathepsin G-regulated release of formyl peptide receptor agonists modulate neutrophil effector functions. PMID: 22879591
  5. Cathepsin G and neutrophil elastase contribute to lung-protective immunity against mycobacterial infections in mice. PMID: 22461690
  6. This is the first study showing a contribution of neutrophil-derived neutral serine proteases CG and NE to lung-protective immunity against focal pneumonia-inducing serotype 19 S. pneumoniae in mice. PMID: 21911460
  7. In the absence of a pathogen challenge, neutrophil-derived proteases and nucleosomes contribute to large vessels thrombosis, the main trigger of myocardial infarction and stroke. PMID: 20676107
  8. Inhibition of TGF-beta significantly reduces microvessel density in mammary tumor-induced bone lesions, mediated by decreased expression of both vascular endothelial growth factor (VEGF) and monocyte chemotactic protein (MCP)-1. PMID: 19646811
  9. neutrophil elastase and cathepsin G are inhibited by PAI-1 mutants PMID: 15131125
  10. cathepsin G activity at the tumor-bone interface plays an important role in mammary tumor-induced osteolysis through RANKL PMID: 18632634
  11. both Cat-G and PAR(4) play key roles in generating and/or amplifying relapses in ulcerative colitis PMID: 19528350
  12. Cathepsin G and MMP9 were identified as proteases involved in enhanced TGF-beta signaling at the tumor-bone interface of mammary tumor-induced osteolytic lesions. PMID: 19671689
  13. Serine protease required for resistance to microbial infection; contributes to pathogenessis in endotoxic shock PMID: 10714686

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Subcellular Location
Cell surface. Membrane; Peripheral membrane protein.
Protein Families
Peptidase S1 family
Tissue Specificity
In adult, detected only in bone marrow where expression is restricted to a small population of early myeloid cells.
Database Links
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