Recombinant Mouse Collagen alpha-1(IV) chain(Col4a1) ,partial

Code CSB-YP005741MO
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Source Yeast
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Code CSB-EP005741MO
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Source E.coli
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Code CSB-EP005741MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005741MO
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Source Baculovirus
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Code CSB-MP005741MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Col4a1
Uniprot No. P02463
Alternative Names Col4a1Collagen alpha-1(IV) chain [Cleaved into: Arresten]
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.; Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation.
Gene References into Functions
  1. Col4a1 mutation generates vascular abnormalities correlated with neuronal damage in a mouse model of HANAC syndrome PMID: 28057519
  2. Embryo implantation triggers dynamic spatiotemporal expression of the basement membrane toolkit (Col4a1, GPBP, Lamc1, peroxidasin) during uterine reprogramming. PMID: 27619726
  3. the lens and possibly vasculature play important roles in Col4a1-related anterior segment dysgenesis. PMID: 28237965
  4. Mutations in murine Col4a1 and Col4a2 genes affected the balance between lung epithelial progenitors and differentiated cells. Mutations in Col4a1 derived from the vascular component were sufficient to cause defects in vascular development and the blood-gas barrier. Col4a1 and Col4a2 mutants displayed disrupted myofibroblast proliferation, differentiation and migration. PMID: 27412481
  5. This extensive description of the muscular phenotype of the Col4a1 HANAC murine model suggests a potential contribution of primary endothelial cell defects, together with muscle BM alterations, to the development of COL4A1-related myopathy. PMID: 28056338
  6. data show that both basement membrane defects and ER stress contribute to Col4a1 renal disease, which has important implications for the development of treatment strategies for collagenopathies PMID: 26839400
  7. Silencing the Col4-alpha1 gene or disrupting integrin engagement by blocking the antibody reduced the expression of platelet-derived growth factor A (PDGF-A), a potent chemotactic factor for fibroblasts. PMID: 25686533
  8. Col4a1 mutations cause abnormal vascular development, which triggers small-vessel disease, recurrent hemorrhagic strokes, and age-related macroangiopathy. PMID: 25753534
  9. Down-regulation let-7 by transforming growth factor-beta1-induced Lin28 upregulates collagen expression in glomerular mesangial cells from diabetic mice. PMID: 25354942
  10. COL4A1 and COL4A2 mutations are pleiotropic and cause a wide spectrum of disorders, including ocular dysgenesis, brain malformations and myopathy, of variable severity in both mice and humans. PMID: 24203695
  11. WT1 maintains testicular cord integrity by additively regulating the expression of basal lamina components Col4a1 and Col4a2 with SOX9. PMID: 23325811
  12. show that heterozygous Col4a1 mutant mice have ocular dysgenesis, neuronal localization defects, and myopathy characteristic of Muscle-eye-brain disease/Walker-Warburg syndrome PMID: 21625620
  13. Data suggest that podocyte von Hippel-Lindau protein is required for normal maintenance of podocytes, basment membrane collagen {alpha}1{alpha}2{alpha}1(IV) deposition and ultrastructure, neuroglobin experssion and glomerular barrier properties. PMID: 20522651
  14. Collectively, our results suggest that vascular basement membrane components substantially impact gene expression in astrocytes during brain tissue repair. PMID: 20091789
  15. Mutations in COL4A1 result in a complex vascular phenotype encompassing defects in maintenance of vascular tone, endothelial cell function and blood pressure regulation. PMID: 20056676
  16. Collagen type IV and Perlecan exhibit dynamic localization in the Allantoic Core Domain. PMID: 19924818
  17. In murine embryos, collagen IV subunits alpha1(IV), alpha2(IV), alpha5(IV) and alpha6(IV) were detected in the basement membrane surrounding the lens vesicle, and they persisted in the capsule until adulthood. PMID: 12225806
  18. findings show that mutations in Col4a1 can lead to perinatal cerebral hemorrhage and can predispose to porencephaly; propose that Col4a1 mutations conspire with environmental trauma in causing the disease PMID: 15905400
  19. Dominant mutations of Col4a1 result in basement membrane defects which lead to anterior segment dysgenesis and glomerulopathy. PMID: 16159887
  20. In this report, we show that a mutation in the mouse Col4a1 gene, encoding procollagen type IV alpha1, predisposes both newborn and adult mice to intracerebral hemorrhage. PMID: 16598045
  21. The genetic, molecular, and phenotypic characterization of nine Col4a1 and three Col4a2 missense mutations recovered in random mutagenesis experiments in the mouse, are presented. PMID: 17179069
  22. Results show that a mutation in Col4a1 gene can cause both ocular anterior segment dysgenesis and optic nerve hypoplasia. PMID: 17317786
  23. Foxc2 plays an important role in the development of the mesenchyme through the regulation of MyoD gene expression but does not regulate Collagen type IV alpha 1 (Col4a1) or Col4a2 PMID: 17506979

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Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane.
Protein Families Type IV collagen family
Tissue Specificity Detected in the basement membrane of the cornea (at protein level).
Database Links

KEGG: mmu:12826

STRING: 10090.ENSMUSP00000033898

UniGene: Mm.738

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