Recombinant Mouse Collagen alpha-2(IV) chain(Col4a2),partial

Code CSB-YP005742MO
Size US$2959
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Col4a2
Uniprot No. P08122
Research Area others
Species Mus musculus (Mouse)
Source Yeast
Expression Region 1481-1707aa
Target Protein Sequence VSIGYLLVKHSQTDQEPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAGSCLARFSTMPFLYCNPGDVCYYASRNDKSYWLSTTAPLPMMPVAEEEIKPYISRCSVCEAPAVAIAVHSQDTSIPHCPAGWRSLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTCHYFANKYSFWLTTIPEQNFQSTPSADTLKAGLIRTHISRCQVCMKNL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 41.1 kDa
Protein Length Partial
Tag Info N-terminal 6xHis-sumostar-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.
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Citations

Canstatin modulates L-type calcium channel activity in rat ventricular cardiomyocytes. KeisukeImoto.et al,Biochemical and Biophysical Research Communications,2018

Application: Drug related studies
Review: Acute effects of recombinant canstatin on the enhanced ICaL induced by COL4A2 siRNA in ventricular cardiomyocytes. Cardiomyocytes isolated from ventricles of COL4A2 siRNA-injected rats for 48 h were treated with canstatin (250 ng/ml) or solvent for 5-10 min.
PMID: 29626474

Canstatin inhibits isoproterenol-induced apoptosis through preserving mitochondrial morphology in differentiated H9c2 cardiomyoblasts.. Okada M.et al,Apoptosis.,2016

Application: In vitro bioactivity in cell culture
Sample type: H9c2 cardiomyoblasts
Species: mouse
Review: After the stimulation with isoproterenol (100 lM) for 48 h in the presence or absence of canstatin (250 ng/ml, 30 min pretreatment),
differentiated H9c2 cardiomyoblasts were treated with 100 nM Mito Tracker Red for 30 min to observe mitochondrial morphology and membrane potential.
PMID: 27315818

Canstatin inhibits hypoxia-induced apoptosis through activation of integrin/focal adhesion kinase/Akt signaling pathway in H9c2 cardiomyoblasts. Hiroki Kanazawa.et al,PLoS One.,2017

Sample type:H9c2 cardiomyoblasts
Sample species:Rat
Application: Drug related studies
Review:Canstatin suppresses hypoxia-induced apoptosis in H9c2 cardiomyoblasts through the activation of αvβ3 and/or αvβ5 integrins. H9c2 cardiomyoblasts were stimulated with hypoxia for 48 h in the presence or absence of canstatin (10±250 ng/ml) and cilengitide (1 μM), an αvβ3 and/or αvβ5 integrin inhibitor.
PMID: 28235037

Target Data

Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Protein Families Type IV collagen family
Database Links

KEGG: mmu:12827

STRING: 10090.ENSMUSP00000033899

UniGene: Mm.181021

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