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Recombinant Mouse Collagen alpha-2 (IV) chain (Col4a2), partial

Recombinant Mouse Collagen alpha-2 (IV) chain (Col4a2), partial

Code	CSB-YP005742MO
MSDS	MSDS
Size	$368
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
Citations
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Col4a2

Uniprot No.

P08122

Research Area

others

Alternative Names

Col4a2Collagen alpha-2(IV) chain [Cleaved into: Canstatin]

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

1481-1707aa

Target Protein Sequence

VSIGYLLVKHSQTDQEPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAGSCLARFSTMPFLYCNPGDVCYYASRNDKSYWLSTTAPLPMMPVAEEEIKPYISRCSVCEAPAVAIAVHSQDTSIPHCPAGWRSLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTCHYFANKYSFWLTTIPEQNFQSTPSADTLKAGLIRTHISRCQVCMKNL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

41.1 kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-sumostar-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Citations

Canstatin modulates L-type calcium channel activity in rat ventricular cardiomyocytes KeisukeImoto.et al,Biochemical and Biophysical Research Communications,2018
Canstatin inhibits hypoxia-induced apoptosis through activation of integrin/focal adhesion kinase/Akt signaling pathway in H9c2 cardiomyoblasts Kanazawa H.et al,PLoS One.,2017
Canstatin inhibits isoproterenol-induced apoptosis through preserving mitochondrial morphology in differentiated H9c2 cardiomyoblasts. Okada M.et al,Apoptosis.,2016

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 3 reviews

Submit a Review here

Applications : Drug related studies

Review: Canstatin suppresses hypoxia-induced apoptosis in H9c2 cardiomyoblasts through the activation of αvβ3 and/or αvβ5 integrins. H9c2 cardiomyoblasts were stimulated with hypoxia for 48 h in the presence or absence of canstatin (10±250 ng/ml) and cilengitide (1 μM), an αvβ3 and/or αvβ5 integrin inhibitor.

By Anonymous

Applications : In vitro bioactivity in cell culture

Review: After the stimulation with isoproterenol (100 lM) for 48 h in the presence or absence of canstatin (250 ng/ml, 30 min pretreatment), differentiated H9c2 cardiomyoblasts were treated with 100 nM Mito Tracker Red for 30 min to observe mitochondrial morphology and membrane potential.

By Anonymous

Applications : Drug related studies

Review: Acute effects of recombinant canstatin on the enhanced ICaL induced by COL4A2 siRNA in ventricular cardiomyocytes. Cardiomyocytes isolated from ventricles of COL4A2 siRNA-injected rats for 48 h were treated with canstatin (250 ng/ml) or solvent for 5-10 min.

By Anonymous

Target Background

Function

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.; Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.

Gene References into Functions

alpha2(IV)NC1 domain has a role in regulating tumor cell behavior PMID: 15743801
These results indicate that SOX9 is essential for Col4a2 expression in mesangial cells and might be involved in the accumulation of alpha2(IV) collagen in experimental nephritis. PMID: 17525254

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

Protein Families

Type IV collagen family

Database Links

KEGG: mmu:12827

STRING: 10090.ENSMUSP00000033899

UniGene: Mm.181021

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