Recombinant Mouse Complexin-3 (Cplx3)

Code CSB-YP844433MO
MSDS
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Source Yeast
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Code CSB-EP844433MO
MSDS
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Source E.coli
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Code CSB-EP844433MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP844433MO
MSDS
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Source Baculovirus
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Code CSB-MP844433MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Cplx3Complexin-3; Complexin III; CPX III
Species
Mus musculus (Mouse)
Expression Region
1-155
Target Protein Sequence
MAFMVKSMVG GQLKNLTGSL GGGEDKGDGD KSAAEAQGMS REEYEEYQKQ LVEEKMERDA QFTQRKAERA TLRSHFRDKY RLPKNETDES QIQLAGGDVE LPRELAKMIE EDTEEEEDKA SVLGQLASLP GLDLSSLKDK AQTTLGDLKQ SAEKC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Complexin that regulates SNARE protein complex-mediated synaptic vesicle fusion. Required for the maintenance of synaptic ultrastructure in the adult retina. Positively regulates synaptic transmission through synaptic vesicle availability and exocytosis of neurotransmitters at photoreceptor ribbon synapses in the retina. Suppresses tonic photoreceptor activity and baseline 'noise' by suppression of Ca(2+) vesicle tonic release and the facilitation of evoked synchronous and asynchronous Ca(2+) vesicle release.
Gene References into Functions
  1. This study links Cplx3 expression with synapse and circuit function in a specific retinal pathway and reveals a role for asynchronous release in circuit gain control. PMID: 27239031
  2. This study demonistrated that Cplx3-positive cells are generated during the 2-day peak of subplate neurogenesis (E11.5-E12.5). survive preferentially after the first postnatal week compared with other subplate neurons. PMID: 22628460
  3. The novel CPXs III and IV may contribute to the unique release efficacy of retinal sensory neurons.(cOMPLEXIN III, MOUSE) PMID: 15911881
Subcellular Location
Cell junction, synapse. Cell membrane; Lipid-anchor.
Protein Families
Complexin/synaphin family
Tissue Specificity
Present in many brain regions, including hippocampus and cerebellum (at protein level). Expressed in the retina (at protein level). Expressed in retinal amacrine cells (at protein level). Expressed in retinal photoreceptor ribbon synapses. Expressed in th
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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