Recombinant Mouse Cytochrome P450 2C29 (Cyp2c29)

Code CSB-YP714494MO
MSDS
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Source Yeast
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Code CSB-EP714494MO
MSDS
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Source E.coli
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Code CSB-EP714494MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP714494MO
MSDS
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Source Baculovirus
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Code CSB-MP714494MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cyp2c29
Uniprot No.
Alternative Names
Cyp2c29Cytochrome P450 2C29; EC 1.14.14.1; Aldehyde oxygenase; CYPIIC29; Cytochrome P-450 MUT-2
Species
Mus musculus (Mouse)
Expression Region
1-490
Target Protein Sequence
MDLVVFLALT LSCLILLSLW RQSSGRGKLP PGPTPLPIIG NFLQIDVKNI SQSFTNFSKA YGPVFTLYLG SKPTVILHGY EAVKEALIDR GEEFAGRGSF PMAEKIIKGF GVVFSNGNRW KEMRRFTLMT LRNLGMGKRN IEDRVQEEAQ CLVEELRKTK GSPCDPTFIL SCAPCNVICS IIFQNRFDYK DKEFLILMDK INENVKILSS PWLQVCNSFP SLIDYCPGSH HKIVKNFNYL KSYLLEKIKE HKESLDVTNP RDFIDYYLIK QKQVNHIEQS EFSLENLAST INDLFGAGTE TTSTTLRYAL LLLLKYPDVT AKVQEEIDRV VGRHRSPCMQ DRSHMPYTDA MIHEVQRFID LLPTSLPHAV TCDIKFRKYL IPKGTTVITS LSSVLHDSKE FPNPEMFDPG HFLNGNGNFK KSDYFMPFST GKRICAGEGL ARMELFLILT TILQNFKLKS LVHPKEIDIT PVMNGFASLP PPYQLCFIPL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
A cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of 14,15 double bond of (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) forming 14,15-epoxyeicosatrienoic acid (14,15-EET) regioisomer. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH--hemoprotein reductase).
Gene References into Functions
  1. Gene-targeted knockdown of CYP2C55, the homologous gene of CYP2C9, demonstrated viability rescue to light-induced cell death, whereas stable expression of functional CYP2C9-GFP fusion protein further exacerbated light-induced cell death. PMID: 24519941
  2. CYP2C29 synthesizes EETs to mediate SSID, and simultaneously releases superoxide and sequential H(2)O(2), which in turn impair SSID. PMID: 22708815
  3. Midlozam metabolism in Cyp3a knockout mice can be attributed to up-regulated Cyp2c enzymes. PMID: 18156313
  4. Report role of CYP2c29/CYP4a in A2A AR-mediated relaxation using A2A AR-null and wild-type mice. PMID: 18805895
Subcellular Location
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Protein Families
Cytochrome P450 family
Tissue Specificity
Expressed in liver as well as in extrahepatic tissues including brain, kidney, lung, heart, and intestine.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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