Recombinant Mouse DNA polymerase eta (Polh)

Code CSB-YP881322MO
MSDS
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Source Yeast
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Code CSB-EP881322MO
MSDS
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Source E.coli
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Code CSB-EP881322MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP881322MO
MSDS
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Source Baculovirus
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Code CSB-MP881322MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Polh
Uniprot No.
Alternative Names
Polh; Rad30a; Xpv; DNA polymerase eta; EC 2.7.7.7; RAD30 homolog A; Xeroderma pigmentosum variant type protein homolog
Species
Mus musculus (Mouse)
Expression Region
1-694
Target Protein Sequence
MAPGQNRVVA LVDMDCFFVQ VEQRQNPHLR NKPCAVVQYK SWKGGGIIAV SYEARAFGVT RNMWADDAKK LCPDLLLAQV RESRGKANLT KYREASVEVM EIMSYFAVIE RASIDEAYID LTSAVQERLQ KLQGQPISAD LLPSTYIEGL PRGPTVEETV QKEAIRKQGL LQWLDSLQSD DPTSPDLRLT VGAMIVEEMR AAIESKTGFQ CSAGISHNKV LAKLACGLNK PNRQTLVSHG SVPQLFSQMP IRKIRSLGGK LGASVIEVLG IEYMGDLTQF TESQLQSHFG EKNGSWLYAM CRGIEHDPVK PRQLPKTIGC SKNFPGKTAL ATREQVQWWL LQLALELEER LTKDRNDNDR VATQLVVSIR FQGDRRLSSL RRCCALPRYD AHKMSQDAFA AIRNCNTSGI QTEWSPPLTM LFLCATKFSA AAPPACTDIT AFLSSDSSCQ PKVPIASSET RTQGSGPAVP TSKEAATSLA SFFQKAAKKQ RMKETSFVPL NTATEKLSSK PSLVFQSSQT TGSQSFFKQK SLLLQHTQLS NSAAPDPPQA SPAAQPSCLP AECVDSGPDD GAVKPVSSKA VSTEMNVAGD SPNVLDSPAY NSQEVTQRAT EDQVLCEKCD SLVPVWDMPE HTDYHFALEL QKSFLQPCTS KPQAIPAVSP QGKRNPKSPS ASSSKRLRPH GMQTLESFFK PLTH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine. Particularly important for the repair of UV-induced pyrimidine dimers. Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis. Targets POLI to replication foci.
Gene References into Functions
  1. the functions of REV3L in maintaining cell viability, embryonic viability and genomic stability are directly dependent on its polymerase activity, and cannot be ameliorated by an additional deletion of pol eta. PMID: 26727495
  2. XP-V has a role in adipose tissue senescence and metabolic syndrome PMID: 26240351
  3. Increase in UV-induced mutations at both G:C and A:T pairs associated with PolH deficiency suggests that PolH contributes to accurate translesion synthesis (TLS) past both T- & C-containing dimers. PMID: 25733082
  4. POLH deficiency did not affect the frequency and patterns of C:G mutations and UNG POLH double deficiency showed an additive effect of single deficiency. PMID: 22960197
  5. REV1 and Polkappa are involved in DNA damage tolerance via Poleta-REV1 interaction when Poleta fails to bypass its cognate substrates. PMID: 22244149
  6. These results reveal genetic interactions between REV1 catalytic activity and POLH and identify an alternative pathway in the generation of C to G and G to C transversions. PMID: 22223762
  7. these data support the existence of PCNA ubiquitination-dependent and -independent activation pathways of Poleta during somatic hypermutation and DNA damage tolerance. PMID: 21889916
  8. inaccurate DNA synthesis by mammalian DNA polymerase eta (pol eta) contributes to somatic hypermutation (SHM) of Ig genes PMID: 12119399
  9. suggest the involvement of the Pol eta and Pol iota proteins in UV-induced skin carcinogenesis PMID: 17015482
  10. results demonstrate that POLH is a limiting factor for A:T mutations and contributes to the efficient diversification of Ig genes and affinity maturation of antibodies PMID: 18825746
  11. observe upon the simultaneous knockdown of Pols kappa and zeta implicates a highly error-free role of Poleta in TLS opposite cyclobutane pyrimidine dimers in mammalian cells. PMID: 19822754
  12. DNA polymerase fidelity and specificity PMID: 11554790

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Subcellular Location
Nucleus.
Protein Families
DNA polymerase type-Y family
Tissue Specificity
Ubiquitous.
Database Links
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