Recombinant Mouse DNA polymerase zeta catalytic subunit (Rev3l), partial

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

Rev3l

Uniprot No.

Alternative Names

Rev3l; Polz; Sez4DNA polymerase zeta catalytic subunit; EC 2.7.7.7; Protein reversionless 3-like; REV3-like; Seizure-related protein 4

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Catalytic subunit of the DNA polymerase zeta complex, an error-prone polymerase specialized in translesion DNA synthesis (TLS). Lacks an intrinsic 3'-5' exonuclease activity and thus has no proofreading function.

Gene References into Functions

Following challenge with AFB1, survival of mouse cells deficient in pol zeta (Rev3L(-/-)) was significantly reduced relative to Rev3L(+/-) cells or Rev3L(-/-) cells complemented through expression of the wild-type human REV3L PMID: 27849610
A single aspartate mutation in the conserved catalytic site of Rev3L generates a hypomorphic phenotype in vivo and in vitro. PMID: 27481099
the functions of REV3L in maintaining cell viability, embryonic viability and genomic stability are directly dependent on its polymerase activity, and cannot be ameliorated by an additional deletion of pol eta PMID: 26727495
results can be unified by a model in which slowly dividing cells accumulate replication-associated DNA breaks but otherwise survive Rev3L deletion, but functional polzeta is essential for responses requiring rapid proliferation PMID: 23386725
Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric polymerase (Pol) zeta, and Pol kappa PMID: 22859295
DNA polymerase zeta generates tandem mutations in immunoglobulin variable regions PMID: 22615128
Pol zeta was determined to be necessary for proliferation of normal cells using conditions in which Rev3L could be conditionally inactivated by Cre recombinase. PMID: 22319213
REV7 subunit of pol zeta mediated the interaction between REV3 and the REV1 C terminus. PMID: 22303021
In the presence of reduced levels of Rev3, an essential component of Polzeta, tumors exhibited pronounced sensitivity to cisplatin, leading to a significant extension in overall survival of treated recipient mice. PMID: 21068376
found that Rev3 is important for a post-replication repair pathway of helix-distorting [6-4]pyrimidine-pyrimidone photoproducts PMID: 19783229
Involvement of mouse Rev3 in tolerance of endogenous and exogenous DNA damage PMID: 11884603
An essential role for REV3 in cell survival: absence of REV3 induces p53-independent embryonic death PMID: 12051777
data support a central role for DNA polymerase eta in the error-free bypass of UV photoproducts PMID: 14517346
Immortalized mouse cell lines that lack a functional Rev3 gene are hypersensitive to UV irradiation and cisplatin treatment. PMID: 15177183
Pol zeta ablation in B cells impairs the germinal center reaction, class switch recombination, DNA break repair, and genome stability. PMID: 19204108

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Subcellular Location

Nucleus.

Protein Families

DNA polymerase type-B family

Database Links

KEGG: mmu:19714

STRING: 10090.ENSMUSP00000019986

UniGene: Mm.439723

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Recombinant Mouse DNA polymerase zeta catalytic subunit (Rev3l), partial

Product Details

Customer Reviews and Q&A

Target Background

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