Recombinant Mouse Diacylglycerol kinase gamma (Dgkg)

Code CSB-YP852784MO
MSDS
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Source Yeast
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Code CSB-EP852784MO
MSDS
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Source E.coli
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Code CSB-EP852784MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP852784MO
MSDS
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Source Baculovirus
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Code CSB-MP852784MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dgkg
Uniprot No.
Alternative Names
Dgkg; Dagk3; Diacylglycerol kinase gamma; DAG kinase gamma; EC 2.7.1.107; 88 kDa diacylglycerol kinase; Diglyceride kinase gamma; DGK-gamma
Species
Mus musculus (Mouse)
Expression Region
1-788
Target Protein Sequence
MSEEQWVSLS SEEFDQLQKY SEYSSKKIKD VLAEFNEGGS LRQYDPHKPI SYDVFKLFMR AYLEVDLPQP LSTHLFLAFS QKPRQETPDH PKEGASSSEP NVSDYNSDNA AKADEACAPD TESKTTKTQA PSKELEAAAP WEDPGALASS SDAPVVYLKD VVCYLSLMET GRPQDKLEFM FRLYDSDENG LLDQAEMDQI VSQMLHVAQY LEWDPTELRP ILKEMLQGMD YDKDGFVSLQ EWINGGMTTI PLLVLLGMDD SGSKGDGRHA WTLKHFKKPT YCNFCRAMLM GVGKQGLCCI YCKYTVHQRC VSKTIHGCVK TNSKAKRSGE VMQHAWVEGN SSVKCDRCHK SIKCYQSVTA RHCVWCRMTF HRKCELSTVC DGGELKDHIL LPTSICPVSG DRQGGKSDGS VAAKGELVTQ YKIIPSPGTH PLLVLVNPKS GGRQGERILR KFHYLLNPEQ VFNLDNGGPT PGLNFFHDTP DFRVLACGGD GTVGWILDCI DKANFTKHPP VAVLPLGTGN DLARCLRWGG GYEGGSLTKI LKEIEQSPLV MLDRWYLEVM PREEVENGDQ VPYNIMNNYF SIGVDASIAH RFHMMREKHP EKFNSRMKNK LWYFEFGTSE TFAATCKKLH DHIELECDGV EVDLSNIFLE GIAILNIPSM YGGTNLWGET KKNRAVIRES RKSVTDPKEL KCCVQDLSDQ LLEVVGLEGA MEMGQIYTGL KSAGRRLAQC SSVTIRTNKL LPMQVDGEPW MQPQCTIKIT HKNQAPMMMG PPQKSSFFSL RRKSRSKD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Has no apparent specificity with regard to the acyl compositions of diacylglycerol. Specifically expressed in the cerebellum where it controls the level of diacylglycerol which in turn regulates the activity of protein kinase C gamma. Through protein kinase C gamma, indirectly regulates the dendritic development of Purkinje cells, cerebellar long term depression and ultimately cerebellar motor coordination.
Gene References into Functions
  1. A novel morphological function of the C-terminal DGK-gamma catalytic region in filopodia formation, independent of its enzymatic activity. PMID: 23054194
  2. DGKgamma functions through its catalytic action as an upstream suppressor of Rac1 and has a role in lamellipodium/ruffle formation PMID: 15102830
Subcellular Location
Membrane. Cytoplasm, cytosol. Cytoplasm, cytoskeleton.
Protein Families
Eukaryotic diacylglycerol kinase family
Tissue Specificity
Expressed in hippocampus and cerebellar Purkinje cells and weakly and diffusely expressed in the granule cells.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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