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Recombinant Mouse Diacylglycerol kinase theta (Dgkq), partial

Recombinant Mouse Diacylglycerol kinase theta (Dgkq), partial

Code	CSB-YP744055MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP744055MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP744055MO-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP744055MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP744055MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Dgkq

Uniprot No.

Q6P5E8

Alternative Names

DgkqDiacylglycerol kinase theta; DAG kinase theta; EC 2.7.1.107; Diglyceride kinase theta; DGK-theta

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Within the adrenocorticotropic hormone signaling pathway, produces phosphatidic acid which in turn activates NR5A1 and subsequent steroidogenic gene transcription. Also functions downstream of the nerve growth factor signaling pathway being specifically activated in the nucleus by the growth factor. Through its diacylglycerol activity also regulates synaptic vesicle endocytosis.

Gene References into Functions

Results suggest that DGKtheta; may play important physiological roles not only in the brain, but also in diverse organs and tissues during the embryonic stages. PMID: 24079595
Diacylglycerol kinase theta is regulated by magnesium and zinc. PMID: 23266086

Subcellular Location

Cytoplasm. Cytoplasm, cytosol. Cell membrane. Cell junction, synapse. Cytoplasm, cytoskeleton. Nucleus. Nucleus speckle. Nucleus matrix.

Protein Families

Eukaryotic diacylglycerol kinase family

Tissue Specificity

Widely expressed in all brain regions, including the cortex and hippocampus with a specific expression in neuronal cells (at protein level).

Database Links

KEGG: mmu:110524

STRING: 10090.ENSMUSP00000057859

UniGene: Mm.260921

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