Recombinant Mouse Dual specificity protein phosphatase 10 (Dusp10)

Code CSB-YP866848MO
MSDS
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Source Yeast
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Code CSB-EP866848MO
MSDS
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Source E.coli
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Code CSB-EP866848MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866848MO
MSDS
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Source Baculovirus
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Code CSB-MP866848MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dusp10
Uniprot No.
Alternative Names
Dusp10; Mkp5Dual specificity protein phosphatase 10; EC 3.1.3.16; EC 3.1.3.48; Mitogen-activated protein kinase phosphatase 5; MAP kinase phosphatase 5; MKP-5
Species
Mus musculus (Mouse)
Expression Region
1-483
Target Protein Sequence
MPPSPLDDRV VVALSRPVRP QDLNLCLDSS YLGSASPGSG SHAPVLATAV VTLKAANLTY MPSSSGSARS LNCGCSSTSC CTVATYDKDH QAQTQAIAAG TATTAIGTST TCPANQMVNN NENTGSVLSP SGGVGSPVSG TPKQLASIKI IYPNDLAKKM TKCSKSHLPS QGPVIIDCRP FMEYNKSHIQ GAVHINCADK ISRRRLQQGK ITVLDLISCR EGKDSFKRIF SKEIIVYDEN TNEPSRVTPS QPLHIVLESL KREGKEPLVL KGGLSSFKQN HGNLCDNSLQ LQECREVGGG ASAASSMLPQ SVPTTPDIEN AELTPILPFL FLGNEQDAQD LDTMQRLNIG YVINVTTHLP LYHYEKGLFN YKRLPATDSN KQNLRQYFEE AFEFIEEAHQ CGKGLLIHCQ AGVSRSATIV IAYLMKHTRM TMTDAYKFVK GKRPIISPNL NFMGQLLEFE EDLNNGVTPR ILTPKLMGME TVV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Protein phosphatase involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily. It preferably dephosphorylates p38.
Gene References into Functions
  1. MKP-5 negatively regulates apoptotic signaling in skeletal muscle. During regeneration, MKP-5 downregulation contributes to myofiber survival. PMID: 29047406
  2. MKP-5-mediated regulation of JNK negatively regulates phosphorylation of GRAB, which subsequently controls secretion of IL-6 PMID: 28096466
  3. DUSP10 regulates intestinal epithelial cell growth and colorectal tumorigenesis. PMID: 25772234
  4. In mouse macrophages, MKP-5 down-regulates the release of inflammatory mediators by controlling p38 MAPK activity PMID: 25615285
  5. MAPK phosphotase 5 deficiency contributes to protection against blood-stage Plasmodium yoelii 17XL infection in mice. PMID: 24634491
  6. MKP-5 is an essential negative regulator of the promyogenic actions of the MAPKs and may serve as a target to promote muscle stem cell function in the treatment of degenerative skeletal muscle diseases PMID: 23543058
  7. MKP5 deficiency significantly reduces foam cell formation. PMID: 22683306
  8. MKP5 is crucial to homeostatic regulation of MAPK activation in inflammatory responses. PMID: 22307906
  9. a function for ASC that is distinct from the inflammasome in modulating MAPK activity and chemokine expression and further identify DUSP10 as a novel ASC target. PMID: 21487011
  10. MKP5 has a principal function in both innate and adaptive immune responses, and represents a novel target for therapeutic intervention of immune diseases PMID: 15306813
  11. The results show an earlier unrecognized and non-redundant function of MKP-5 in restraining p38 MAPK-mediated neutrophil oxidant production, thereby preventing lipopolysaccharide-induced vascular injury. PMID: 19696743

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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