Recombinant Mouse ETS-related transcription factor Elf-1 (Elf1)

Code CSB-YP726673MO
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Source Yeast
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Code CSB-EP726673MO
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Source E.coli
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Code CSB-EP726673MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP726673MO
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Source Baculovirus
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Code CSB-MP726673MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Elf1
Uniprot No.
Alternative Names
Elf1ETS-related transcription factor Elf-1; E74-like factor 1
Species
Mus musculus (Mouse)
Expression Region
1-612
Target Protein Sequence
MAAVVQQNDL VFEFASNGME DEQQLGDPAI FPAVIVEHVP GADILNSYAG LACVEEPNDM ITESSLDVAE EEIIDDDDDD ITLTVEASCH NGDETIETIE AAEALLNIDS PSPPVLDEKQ INNNIFSSSE DDIVAPITHV SVTLDGIPEV METQQVQETN ADSPGASSPE QRKRKKGRKT KPPRPDSPTT TPNISVKKKN KDGKGNTIYL WEFLLALLQD KATCPKYIKW TQREKGIFKL VDSKAVSRLW GKHKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK EMPKDLIYID DEDPSSSIES SDQSLSSTTA SSRNQANRSR VSSSPGIKGG AATILKPGNS KAANPKDPVE VGQPSEVLRT VQPSQAPYPT QLFRTVHVVQ PVQAVPEEAT IASTMQEEAA NSSVPSIRTI QASTQVPVVV SPGNQQLHTV TVPLTTVIAS IDPSSGAGSQ KFILQTIPSS QPMTVLKENV MLQSQKPGSP SIVLSPTQVQ QVLTSNVQSI CNGAGSVASA PSFSATTPVV TFSRSSQLVA HPPGTVITSV IKAQETKTLK QEVEKKAEDD LNEDAEKSAQ QPQPYVMVLS SSNGFSSQVA VKQNELLEPN SF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that activates the LYN and BLK promoters.
Gene References into Functions
  1. Green Tea Polyphenol EGCG Upregulates Tollip Expression by Suppressing Elf-1 Expression PMID: 28954885
  2. Herein, the authors demonstrate that mice lacking Sts expression are significantly resistant to infection by the live vaccine strain of Francisella tularensis. PMID: 28630061
  3. Whereas the proportion of NK cells in Elf-1(-/-) mice was normal, the proportion of NKT cells was significantly reduced in the thymus and periphery of Elf-1(-/-) mice compared with wild-type mice. PMID: 21148815
  4. crystal structure of the phosphatase domain of Sts-1, Sts-1(PGM), was determined at pH 4.6.The asymmetric unit contains two independent molecules and each active site is occupied by a sulfate ion. PMID: 20516590
  5. Results suggest that GATA-2, Fli-1 and Elf-1 are key components of an enhanceosome responsible for activating SCL transcription and establishing the transcriptional programme required for HSC formation. PMID: 12065417
  6. These results suggest that several Ets factors, including Fli1 itself, are involved in the transcriptional regulation of Fli1 in lymphocytes. PMID: 17606295
  7. The Sts-1,2 proteins target tyrosine phosphorylated, ubiquitinated proteins within TCR signaling pathways. PMID: 19733910

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Subcellular Location
Nucleus.
Protein Families
ETS family
Tissue Specificity
Predominantly found in hematopoietic cells. Detected in other cell types such as fibroblasts.
Database Links
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