Recombinant Mouse Endonuclease V (Endov)

Code CSB-YP806983MO
MSDS
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Source Yeast
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Code CSB-EP806983MO
MSDS
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Source E.coli
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Code CSB-EP806983MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP806983MO
MSDS
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Source Baculovirus
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Code CSB-MP806983MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Endov
Uniprot No.
Alternative Names
Endov; Endonuclease V; EC 3.1.26.-
Species
Mus musculus (Mouse)
Expression Region
1-338
Target Protein Sequence
MAHTAAERPP EETLSLWKGE QARLKARVVD RDTEAWQRDP SFSGLQKVGG VDVSFVKGDS VRACASLVVL SYPELKVVYE DSRMVGLKAP YVSGFLAFRE VPFLVELVQR LQEKEPDLMP QVVLVDGNGV LHQRGFGVAC HLGVLTELPC IGVAKKLLQV DGLENNALHK EKIVLLQAGG DTFPLIGSSG TVLGMALRSH DHSTKPLYVS VGHRISLEVA VRLTHHCCRF RIPEPIRQAD IRSREYIRRT LGQLGVAPAQ RKDRSQKEQR PNACPQGGPG ALADQGRPPE CDGRDSSSDR KAPEPGFQEQ KDQQLEGTGH QEDSDLWPPS PAWVQSPP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine. Active against both single-stranded and double-stranded RNAs. Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs). Cleaves mRNAs and tRNAs containing inosine. Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3'. Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing. Binds branched DNA structures.
Gene References into Functions
  1. Mouse Endonuclease V shares substrate preferences with human EndoV by catalyzing cleavage at inosines in RNA and being inert towards inosines in DNA. PMID: 27108838
Subcellular Location
Cytoplasm. Nucleus, nucleolus. Cytoplasm, Stress granule.
Protein Families
Endonuclease V family
Tissue Specificity
Highest levels detected in liver with high levels also found in heart, kidney and testis. Expressed at low levels in brain.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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