Product Details

Purity

>85% (SDS-PAGE)

Target Names

Slc1a6

Uniprot No.

O35544

Alternative Names

Slc1a6; Eaat4Excitatory amino acid transporter 4; High-affinity neuronal glutamate transporter; Sodium-dependent glutamate/aspartate transporter; Solute carrier family 1 member 6

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (Probable).

Gene References into Functions

EAAT4 was downregulated due to the loss of Rheb1 in Purkinje cells; mTORC1 was downregulated and Akt was upregulated in Rheb1 cKO mice, suggesting that mTORC1 and Akt may be related to the downregulation of EAAT4; Rheb1 knockout decreased EAAT4 currents and slowed down the kinetics of AMPA currents; Rheb1 deficiency did not affect the morphology of Purkinje cell layer and the development of Purkinje cells PMID: 26194056
Glutamate transporters EAAT4 and EAAT5 are expressed in vestibular hair cells and calyx endings. PMID: 23049999
The role of EAAT4 in cortical glutamatergic transmission may be more important than previously thought. PMID: 20211693
Purkinje cell loss in the Inpp4a(wbl) mutant is due to glutamate excitotoxicity initiated by the climbing fiber, and that Eaat4 may exert a protective effect. PMID: 20011524
abnormal expression of EAAT4, GABRA6, Spi2 combined with lower levels of glutamate and GABA are likely to be associated with the pathophysiology of Canavan disease. PMID: 12909286
These results demonstrate that EAAT4 is expressed in astrocytes. This astrocytic localization of neuronal EAAT4 may reveal a new function of EAAT4 in the central nervous system. PMID: 12951653
EAAT4 mRNA is expressed in the cerebellum of prion protein-deficient (PrP-/-) mice presenting with cerebellar ataxia, at the levels identical to those in the cerebellum of non-ataxic PrP+/- mice PMID: 14625012
EAAT4 is present in the outer segments, a nonsynaptic region of photoreceptors, where it might provide a feedback mechanism for sensing extracellular glutamate or serve as an outer barrier to prevent glutamate from escaping from the retina. PMID: 15737735
The main role of EAAT4 is to remove low concentrations of glutamate that escape from the uptake by glial transporters at late times and thus prevent the transmitter from spilling over to neighboring synapses. PMID: 16177048
The correlation of promoter activity to protein expression makes the EAAT4 BAC promoter reporter a valuable tool to study regulation of EAAT4 expression. PMID: 17022974
PF-evoked mGluR-EPSCs showed larger amplitude and faster rising kinetics in EAAT4-deficient mice than in the wild-type mice. EAAT4 is most closely involved in mGluR activation in PCs among the glutamate transporters. PMID: 17727989
Slc1a6 is downregulated in staggerer mice and is a direct transcriptional target of RORa PMID: 14687547

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family, SLC1A6 subfamily

Tissue Specificity

Brain specific.

Database Links

KEGG: mmu:20513

STRING: 10090.ENSMUSP00000005490

UniGene: Mm.6257

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Recombinant Mouse Excitatory amino acid transporter 4 (Slc1a6), partial

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