Recombinant Mouse Fascin (Fscn1)

Code CSB-YP726751MO
MSDS
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Source Yeast
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Code CSB-EP726751MO
MSDS
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Source E.coli
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Code CSB-EP726751MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP726751MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP726751MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Fscn1
Uniprot No.
Alternative Names
Fscn1; Fan1; SnlFascin; Singed-like protein
Species
Mus musculus (Mouse)
Expression Region
2-493
Target Protein Sequence
TANGTAEAV QIQFGLISCG NKYLTAEAFG FKVNASASSL KKKQIWTLEQ PPDEAGSAAV CLRSHLGRYL AADKDGNVTC EREVPDGDCR FLVVAHDDGR WSLQSEAHRR YFGGTEDRLS CFAQSVSPAE KWSVHIAMHP QVNIYSVTRK RYAHLSARPA DEIAVDRDVP WGVDSLITLA FQDQRYSVQT SDHRFLRHDG RLVARPEPAT GFTLEFRSGK VAFRDCEGRY LAPSGPSGTL KAGKATKVGK DELFALEQSC AQVVLQAANE RNVSTRQGMD LSANQDEETD QETFQLEIDR DTRKCAFRTH TGKYWTLTAT GGVQSTASTK NASCYFDIEW CDRRITLRAS NGKFVTAKKN GQLAASVETA GDSELFLMKL INRPIIVFRG EHGFIGCRKV TGTLDANRSS YDVFQLEFND GAYNIKDSTG KYWTVGSDSS VTSSSDTPVD FFLEFCDYNK VALKVGGRYL KGDHAGVLKA CAETIDPASL WEY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Actin-binding protein that contains 2 major actin binding sites. Organizes filamentous actin into parallel bundles. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF.
Gene References into Functions
  1. MAGE-G1 interacted with fascin 1 or vimentin in P19 cells after a 6-day retinoic acid-induced neuronal differentiation. PMID: 28374796
  2. The data suggested that Daam1 affected actin assembly during oocyte meiotic division via the regulation of fascin expression. PMID: 28682694
  3. Filopodia formation by crosslinking of F-actin with fascin in two different binding manners PMID: 27169557
  4. Fascin-1 expression, cadherin-17 expression, tumor size, and differentiation were independent risk factors for GC. PMID: 26743780
  5. Fascin1 facilitates phagolysosomal fusion for killing of phagocytosed Listeria. It binds to LC3, an autophagosome marker, both in vivo & in vitro. It plays a critical role in dendritic cell survivalduring Listeria infection. PMID: 24244012
  6. A tightly regulated phospho/dephospho-fascin cycle modulated by extracellular signals is required for the polarized morphology and migration in neuroblasts, thus contributing to efficient neurogenesis. PMID: 23884926
  7. fascin 1 promotes cell migration and has a role in growth and cell cycle progression PMID: 23633513
  8. Suppression of fascin expression results in reduction of activation-induced migration and invasion in mouse monocytic leukemia RAW264.7 macrophages. PMID: 22102721
  9. Both STAT3 and NFkappaB are required for the cytokine-induced expression of fascin in cancer cells. PMID: 21937440
  10. Fascin1 is critical for cell migration; expression of exogenous fascin1 in fascin1-null dendritic cells (DC) rescues the defects in membrane protrusive activity, as well as in DC podosome disassembly. PMID: 21263068
  11. fascin-1 is principally expressed in neural and mesenchymal derivatives during embryonic development PMID: 15465486
  12. We propose that fascin is a key specific actin cross-linker, providing stiffness for filopodial bundles, and that its dynamic behavior allows for efficient coordination between elongation and bundling of filopodial actin filaments. PMID: 16966425
  13. Fascin mRNA & protein levels were significantly lower in the cultured ANXA2(-/-) cells than in cultured ANXA2(+/+) cells. ANXA2 may be an inducer or enhancer of fascin expression at the transcription level. PMID: 18608216
  14. The results indicate that fascin1-mediated filopodia assembly is dispensable for mouse development. PMID: 19343791
  15. study found that Rab35 regulates the assembly of actin filaments in filopodia formation; effect was mediated by the actin-bundling protein fascin, which directly associated with active Rab35 PMID: 19729655

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Subcellular Location
Cytoplasm, cytosol. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber. Cell projection, growth cone. Cell projection, filopodium. Cell projection, invadopodium. Cell projection, microvillus. Cell junction.
Protein Families
Fascin family
Tissue Specificity
Most abundant in brain. Detected at lower levels in lung, uterus, small intestine and spleen (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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