Product Details

Purity

>85% (SDS-PAGE)

Target Names

Foxe3

Uniprot No.

Q9QY14

Alternative Names

Foxe3Forkhead box protein E3

Species

Mus musculus (Mouse)

Expression Region

1-288

Target Protein Sequence

MDAQVAFSGF PALPSLTPSG PQLPTLAGAE PGREPEEVVG GGDAEPTAVP GPGKRRRRPL QRGKPPYSYI ALIAMALAHA PGRRLTLAAI YRFITERFAF YRDSPRKWQN SIRHNLTLND CFVKVPREPG NPGKGNYWTL DPAAADMFDN GSFLRRRKRF KRAELPAPPP PPPPFPYAPF PPPPAPASAP PARLFRLDSL LGLQPEPPGP VAPEPPCCAA PDAAFPPCAA AASPPLYSPA SERLGLPAPL PAQPLLALAG SAGALGPLGA GEAYLRQPGF APGLERYL

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Transcription factor that controls lens epithelial cell growth through regulation of proliferation, apoptosis and cell cycle. During lens development, controls the ratio of the lens fiber cells to the cells of the anterior lens epithelium by regulating the rate of proliferation and differentiation. Controls lens vesicle closure and subsequent separation of the lens vesicle from ectoderm. Is required for morphogenesis and differentiation of the anterior segment of the eye. Controls the expression of DNAJB1 in a pathway that is crucial for the development of the anterior segment of the eye.

Gene References into Functions

These results clearly show that the development of early-onset cataracts requires at least two mutant alleles of Foxe3(rct) and Pde6b(rd1), and another modifier associated with the severity of cataract phenotypes in Foxe3(rct) mice underlies the genetic backgrounds in mice. PMID: 29317205
The Hedgehog pathway with its member Smoothened is required during a discrete embryonic period (E9.5-E12.5) in lens development to regulate lens epithelial cell proliferation, survival and FoxE3 expression. PMID: 25268479
Foxe3 as a transcriptional target of Pitx3 explains at least in part some of the phenotypic similarities of the ak and dyl mice (dysgenic lens, a Foxe3 allele). PMID: 24307298
The observed changes in the expression of FoxE3 suggest that Msx2 is an important contributor in controlling transcription of target genes critical for early eye development. PMID: 22503753
the ectopic activation of downstream effectors of the hedgehog signaling pathway in the mouse lens disrupts normal fiber cell differentiation by a mechanism consistent with a sustained epithelial cellular developmental program driven by FoxE3. PMID: 22491411
cataracts in rct mice are caused by reduced Foxe3 expression in the lens and this decreased expression is a result of a deletion in a cis-acting regulatory element PMID: 22002806
Foxe3 promoter was activated by Sip1 PMID: 16162653
targeted disruption of Foxe3 results in abnormal development of the eye. Cells of the anterior lens epithelium show a decreased rate of proliferation, resulting in a smaller than normal lens. PMID: 16199865
early Foxe3 expression is sensitive to halved Pax6 gene dosage & there is phenotypic similarity between Pax6 & Foxe3 mutants; we propose that many ocular malformations associated with Pax6 haploinsufficiency are consequences of reduced Foxe3 expression PMID: 17064680
Proper inactivation of FoxE3 expression at the lens equator is important for many aspects of fiber differentiation, and persistent expression leads to a partial epithelialization of fiber cells, with severe consequences for lens function. PMID: 18539941
Data found no evidence that Pitx3 and Foxe3 genetically interact. PMID: 19334279

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Involvement in disease

Defects in Foxe3 are the cause of the dysgenetic lens (dyl) phenotype. In mouse mutant dyl the lens vesicle fails to separate from the ectoderm, causing a fusion between the lens and the cornea. Lack of a proliferating anterior lens epithelium leads to absence of secondary lens fibers and a dysplastic, cataractous lens.

Subcellular Location

Nucleus.

Tissue Specificity

Expressed in the embryonic lens.

Database Links

KEGG: mmu:30923

STRING: 10090.ENSMUSP00000050445

UniGene: Mm.377122

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Recombinant Mouse Forkhead box protein E3 (Foxe3)

Product Details

Customer Reviews and Q&A

Target Background

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