Product Details

Purity

>85% (SDS-PAGE)

Target Names

Gsdma3

Uniprot No.

Q5Y4Y6

Alternative Names

Gsdma3; Gsdm3Gasdermin-A3; Gasdermin-3

Species

Mus musculus (Mouse)

Expression Region

1-464

Target Protein Sequence

MPVFEDVTRA LVRELNPRGD LTPLDSLIDF KHFRPFCLVL RKRKSTLFWG ARYVRTDYTL LDLLEPGSSP SDLTDSGNFS FKNMLDVQVQ GLVEVPKTVK VKGTAGLSQS STLEVQTLSV APSALENLKK ERKLSADHSF LNEMRYHEKN LYVVMEAVEA KQEVTVEQTG NANAIFSLPS LALLGLQGSL NNNKAVTIPK GCVLAYRVRL LRVFLFNLWD IPYICNDSMQ TFPKIRRVPC SAFISPTQMI SEEPEEEKLI GEMHEDFKTL KEEVQRETQE VEKLSPVGRS SLLTSLSHLL GKKKELQDLE QKLEGALDKG QKVTLEALPK DVLLSKDAMD AILYFLGALT ELTEEQLKIL VKSLEKKILP VQLKLVESTL EQNFLQDKEG VFPLQPDLLS SLGEEELTLT EALVGLSGLE VQRSGPQYAW DPDTRHNLCA LYAGLSLLHL LSRKSNALTY CALS

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Precursor of a pore-forming protein involved in the transition from catagen to telogen at the end of hair follicle morphogenesis. This form constitutes the precursor of the pore: upon cleavage, the released N-terminal moiety (Gasdermin-A3, N-terminal) binds to membranes and forms pores, triggering cell death.; Pore-forming protein that causes membrane permeabilization and pyroptosis. Released upon cleavage in vitro of genetically engineered Gsdma3, and binds to membrane inner leaflet lipids. Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis. Binds to membrane inner leaflet lipids, including bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to monophosphorylated phosphatidylinositols. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity. The functional mechanisms and physiological proteases that cleave and activate this pore-forming protein are unknown (Probable). Plays a role in the transition from catagen to telogen at the end of hair follicle morphogenesis, possibly by regulating hair follicle stem cell niche maintenance. Also required for mammary gland development.

Gene References into Functions

cryo-electron microscopy structures of the 27-fold and 28-fold single-ring pores formed by the N-terminal fragment of mouse GSDMA3 (GSDMA3-NT) at 3.8 and 4.2 A resolutions, and of a double-ring pore at 4.6 A resolution PMID: 29695864
In this study, we describe a novel mutation (C+/H-: cornea plus, hair minus) in the far C-terminal region of Gasdermin A3 that causes both skin and corneal phenotypes. PMID: 29372254
The function of Gsdma3 in regulating the development of mammary glands, and it demonstratse that the Gsdma3 gene may act as a suppressor of the immune reaction. PMID: 28168650
induced expression caused epidermal hyperplasia, skin inflammation and hair cycle defect, suggesting gain-of-function mutations PMID: 26173759
crystal structure of GSDMA3 showed an autoinhibited two-domain architecture that is conserved in the gasdermin family; the gasdermin-N domain is required for pyroptosis PMID: 27281216
Our results indicate that the genetic mutations in the C-terminal domain of Gsdma3 are gain-of-function mutations which unmask the N-terminal functional domain of Gsdma3 PMID: 26100518
GSDMA3 mutation play a specific role in hair growth in alopecia. PMID: 25860385
Loss of conserved Gsdma3 self-regulation causes autophagy and cell death. PMID: 25825937
The Gsdma3 may play a role in the proliferation and differentiation of epidermal cells and hair follicles through negatively regulating beta-catenin expression. PMID: 22694914
in mouse skin keratinocytes, Gsdma3 expression could be regulated by TNF-alpha. PMID: 22585037
Gsdma3(I359N) is a novel ENU-induced mutant mouse line for studying the function of Gasdermin A3 in the hair follicle and epidermis PMID: 22682752
The results demonstrate that Gsdma3 causes immune-mediated destruction of bulge stem cells plays a crucial role in the pathogenesis of alopecia in AE mice, and this strain might be implicated in other PCAs including lichen planopilaris. PMID: 22155111
Gsdma3 gene mutation interfered with catagen formation during mouse hair follicle cycle and, by upregulation of Caspase-3 expression and promotion of apoptosis, Gsdma3 gene could play an essential role in normal catagen induction. PMID: 21805153
These results suggest that Gsdma3 is necessary in hair shaft differentiation, and involved in Msx2 regulatory pathway. PMID: 20977888
Gsdm3 reflects a mutation hotspot and that Gsdm3 mutations cause alopecia in Rco2, Re(den), and Bsk mice. PMID: 15475261
role for gasdermins in differentiation of the epidermis and its appendages. PMID: 15737203
These results suggest that Gsdma3 is involved in the proliferation and differentiation of epidermal stem cells. PMID: 17572385

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Involvement in disease

Defects in Gsdma3 are the cause of a number of alopecia phenotypes, bareskin (Bsk), defolliculated (Dfl), finnegan (Fgn) reduced coat 2 (Rco2), Rex-denuded (Re-den) and recombination induced mutation 3 (Rim3). These are dominant conditions characterized by loss of hair.

Subcellular Location

[Gasdermin-A3]: Cytoplasm, cytosol.; [Gasdermin-A3, N-terminal]: Cell membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein.

Protein Families

Gasdermin family

Tissue Specificity

Highest levels in skin with weak expression in placenta and testis. Not detected in the gastrointestinal tract. In skin, expressed in postnatal hair follicles and epidermis as well as sebaceous gland basal cells.

Database Links

KEGG: mmu:450219

UniGene: Mm.371065

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Recombinant Mouse Gasdermin-A3 (Gsdma3)

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