Recombinant Mouse Girdin (Ccdc88a), partial

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

Ccdc88a

Uniprot No.

Alternative Names

Ccdc88a; Grdn; Kiaa1212; Girdin; Akt phosphorylation enhancer; APE; Coiled-coil domain-containing protein 88A; G alpha-interacting vesicle-associated protein; GIV; Girders of actin filament; Hook-related protein 1; HkRP1

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Bifunctional modulator of guanine nucleotide-binding proteins (G proteins). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein G(i) alpha subunits. Also acts as a guanine nucleotide dissociation inhibitor for guanine nucleotide-binding protein G(s) subunit alpha GNAS. Essential for cell migration. Interacts in complex with G(i) alpha subunits with the EGFR receptor, retaining EGFR at the cell membrane following ligand stimulation and promoting EGFR signaling which triggers cell migration. Binding to Gi-alpha subunits displaces the beta and gamma subunits from the heterotrimeric G-protein complex which enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB. Phosphorylation of AKT1/PKB induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation. Binds in its tyrosine-phosphorylated form to the phosphatidylinositol 3-kinase (PI3K) regulatory subunit PIK3R1 which enables recruitment of PIK3R1 to the EGFR receptor, enhancing PI3K activity and cell migration. Plays a role as a key modulator of the AKT-mTOR signaling pathway, controlling the tempo of the process of newborn neuron integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Inhibition of G(s) subunit alpha GNAS leads to reduced cellular levels of cAMP and suppression of cell proliferation. Essential for the integrity of the actin cytoskeleton. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. Plays a role in ciliogenesis and cilium morphology and positioning and this may partly be through regulation of the localization of scaffolding protein CROCC/Rootletin.

Gene References into Functions

Tyrosine Phosphorylation of an Actin-Binding Protein Girdin Specifically Marks Tuft Cells in Human and Mouse Gut PMID: 28375676
This study aimed to prove the participation of mTORC2/Akt in F-actin assembling in early-stage cleavage of mouse fertilized eggs via the function of Girdin. PMID: 27666957
Akt-dependent Girdin phosphorylation regulates repair processes after acute myocardial infarction. PMID: 26393439
GIV expression is up-regulated in liver after fibrotic injury and is required for hepatic stellate cells activation.Girdin is a central hub for profibrogenic signalling networks during liver fibrosis. PMID: 25043713
GIV and its substrate Galphai3 are recruited to active integrin complexes PMID: 26391662
findings suggest a potential role of Girdin/kinesin-1 interaction in the regulation of neuroblast migration in the postnatal brain PMID: 26072378
Girdin phosphorylation at Y1798 was found at focal adhesions in NIH3T3 cells. Such phosphorylation was found where migration defects occurred in Girdin KO mice. PMID: 25707853
Girdin phosphorylation at S1416 induced by Akt is crucial for NMDA receptor activation associated with LTP. PMID: 25378165
Girdin was identified as a new and major regulator of the insulin signal in myoblasts and skeletal muscle. PMID: 23886629
These results demonstrate that girdin and its phosphorylation play an important role in neonatal vascular development and in pathological neovascularization in the retina. PMID: 23195430
these findings indicate that loss of Girdin in the nestin cell lineage underlies the phenotype of Girdin ncKO mice. PMID: 22974978
Girdin regulates cell movement in biological contexts that require directional cell movement PMID: 22574214
Girdin and its Akt-mediated phosphorylation have major roles in the migration and proliferation of vascular smooth muscle cells and vascular remodeling. PMID: 21415395
A structural determinant that renders G alpha(i) sensitive to activation by GIV/girdin is required to promote cell migration. PMID: 20157114
HkRP1 (A430106J12Rik) is involved in the process of tubulation of sorting nexin-1 positive membranes from early endosome subdomains. PMID: 15882442
the Akt/Girdin signalling pathway is essential in VEGF-mediated postneonatal angiogenesis PMID: 18264090
Girdin has an important role in tumor progression with aberrant activation of the Akt signaling pathway PMID: 18316593
Girdin as an intrinsic factor in postnatal development of the dentate gyrus and provide insights into the critical role of the DISC1/girdin interaction in postnatal neurogenesis in the dentate gyrus. PMID: 19778507

Hide All

Subcellular Location

Cell membrane; Peripheral membrane protein. Cytoplasm, cytosol. Cytoplasmic vesicle. Cell projection, lamellipodium. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.

Protein Families

CCDC88 family

Tissue Specificity

Expressed in the dentate gyrus, pyramidal cell layer of hippocampal regions CA1 and CA3 at postnatal 15. Expressed highly in neurons. Weakly in neuron progenitors (at protein level). Expressed in the dentate granule cell layer of the hippocampus. Expresse

Database Links

KEGG: mmu:108686

STRING: 10090.ENSMUSP00000048978

UniGene: Mm.338284

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-YP722540MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP722540MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP722540MO-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP722540MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP722540MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Mouse Girdin (Ccdc88a), partial

Product Details

Customer Reviews and Q&A

Target Background

×CloseMessage