Recombinant Mouse Glutamate receptor 1 (Gria1), partial

Code CSB-YP009898MO
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Source Yeast
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Code CSB-EP009898MO
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Source E.coli
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Code CSB-EP009898MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009898MO
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Source Baculovirus
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Code CSB-MP009898MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Gria1
Uniprot No.
Alternative Names
Gria1; Glur1Glutamate receptor 1; GluR-1; AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; Glutamate receptor ionotropic; AMPA 1; GluA1
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
Gene References into Functions
  1. The nucleus-accumbens-specific knockdown of RGS4 significantly increased the behaviors associated with morphine and did so by phosphorylation of the GluR1 (Ser831) and NR2A (Tyr1325) glutamate receptors in the NAc. PMID: 29754475
  2. Data indicate a key role of FoxO3a/Zdhhc3/GluA1 axis in the high-fat diet (HFD)-dependent impairment of cognitive function. PMID: 29222408
  3. translocation of surface GluA1, but not GluA2, AMPAR subunits to the synapse requires the amino-terminal domain PMID: 28630296
  4. Taken together, these results suggest that ApoE4 enhances Abeta inhibition of insulin-stimulated AMPA receptor function, which accelerates memory impairment in ApoE4xAPP mice. PMID: 27189808
  5. Both hippocampal as well as extra-hippocampal GluA1-containing AMPA receptors contribute to spatial working memory. PMID: 27417577
  6. Presynaptic (CA3 pyramidal cell), but not postsynaptic (CA1 pyramidal cell), deletion of N-methyl-d-aspartate (NMDA)-type glutamate receptors eliminated the ketamine-induced enhancement of excitatory synaptic transmission in hippocampal slices and the antidepressant actions of ketamine PMID: 27965425
  7. In the present study, by combining a 64-channel multielectrode system and a novel analysis and visualization method, we observed the accurate spatial localization and dynamic temporal changes of network fEPSP signals and LTP responses within the ACC circuit and found that PKA phosphorylation, but not PKC phosphorylation, of the GluA1 is required for LTP in the ACC. PMID: 28765333
  8. Data suggest that phosphorylation of Shp2/Ptpn11 at Tyr542 and its translocation to postsynaptic compartment are integral processes in synaptic scaling/homeostasis; Shp2 phosphatase activity is critical to regulation of Ser(P)845 GluA1 and surface expression of GluA1 during synaptic scaling. (Shp2/Ptpn11 = protein tyrosine phosphatase non-receptor type 11; GluA1 = glutamate receptor ionotropic Ampa1 [alpha 1]) PMID: 28768764
  9. Gria1 expression in Purkinje cells is not required for cerebellar motor learning. PMID: 28103481
  10. Three epilepsy-associated missense mutations reduce neural precursor cell expressed developmentally down-regulated gene 4-2 (Nedd4-2)-mediated AMPA receptor GluA1 ubiquitination. PMID: 28212375
  11. Chronic stress-elicited depressive behavior may be due to hypertrophy of basolateral amygdala (BLA) neuronal dendrites and increased of Glur1-Glur2 ratio in BLA neurons. PMID: 28336441
  12. Study demonstrated that naltrexone-induced plasticity in excitatory synapses, via the transitory increase of GluA1 insertion at the postsynaptic density and GluA1-S845 phosphorylation, facilitates learning PMID: 26049209
  13. Although dephosphorylation of Serine 845 is thought to have a key role in long term depression (LTD), results indicate that few GluA1 subunits in hippocampal neurons are phosphorylated at this site. In contrast, approximately 50% of GluA1 subunits are phosphorylated at threonine 840, suggesting that dephosphorylation of this site can contribute to the down-regulation of AMPAR-mediated synaptic transmission in LTD. PMID: 26980779
  14. Information load regulates AMPA-R phosphorylation within the hippocampus, and an overload condition associated with impaired memory is paralleled by a lack of AMPA-R phosphorylation. PMID: 25381005
  15. The findings of this study suggested that phosphorylation of GluA1 at S831 plays an important role in the development of hypersensitivity after SCL. PMID: 26724583
  16. found the protein levels of AMPA receptor subunits (GluR1 and GluR2) are upregulated in the amygdala and the 5-HT3 receptor is downregulated in hypothalamic regions of Socially Isolated mice. PMID: 26522741
  17. hippocampus displayed increased levels of the alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate receptor subunit GluA1 PMID: 25545823
  18. These results provide direct evidence for cortical AMPA receptors to contribute to zymosan-induced visceral and spontaneous pain. PMID: 26585043
  19. These results provide evidence for VPS35's function in promoting spine maturation, which is likely through increasing AMPA receptor targeting to the postsynaptic membrane. PMID: 26521016
  20. Binge alcohol drinking produced reduced GluA1 expression in adolescent amygdala but differentially increased GluA1 in adult amygdala. PMID: 26247621
  21. These results highlight the pivotal role of FUS in regulating GluA1 mRNA stability, post-synaptic function and fronto-temporal lobar degeneration-like animal behaviors. PMID: 25968143
  22. Results show that a loss of GLUA1 protein in 5-HT neurons enhances AMPA receptor function and leads to multiple local molecular and neurochemical changes in the raphe nuclei that dysregulate 5-HT neuronal activity and induce anxiety-like behavior PMID: 25547714
  23. We identified glutamate receptor subunit 1 (GluA1), subunit of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors as a novel substrate of Nedd4-2 PMID: 26250624
  24. GPR30 activation induced anxiolytic effects but did not affect the nociceptive threshold of mice under chronic pain. The anxiolytic effects of GPR30 were partially due to Glur1 upregulation and GABA-A receptor downregulation in the basolateral amygdala. PMID: 25614360
  25. Data indicate that the AMPA receptor subunits abundance is hippocampus, GluA2 > GluA1 > GluA3 >> GluA4; cortex, GluA2 > GluA3 >/= GluA1 >> GluA4; and cerebellum, GluA2 > GluA3 >/= GluA1 > GluA4. PMID: 25337787
  26. Loss of Gria1 is associated with abnormal hyperlocomotion. PMID: 24932798
  27. Results show that LRP1 interacts with and controls the cellular distribution, turnover and phosphorylation of GluA1, which in turn influences calcium influx, neurite outgrowth and filopodia formation in neurons. PMID: 25500815
  28. results suggest that abnormal (hippocampal) glutamatergic transmission underlies the hyperactive phenotype of the Gria1-/- mice in a novel environment PMID: 25446922
  29. Review of behavioural phenotype of Gria1 knockout mice representing a cause of aberrant salience and implications for neural mechanisms of schizophrenia PMID: 25224260
  30. CNTNAP2 has a role in the correct trafficking of GluA1 AMPA-type glutamate receptors PMID: 25918374
  31. This study investigated the effect of temporally and spatially restricted gene manipulation of GluA1 on behavioural correlates of mood disorders in mice. PMID: 24895223
  32. peripheral nerve injury induces postsynaptic GluA1 accumulation in cingulate cortical neurons. PMID: 25359681
  33. data provide new insight into the role of deficient AMPA receptors specifically during late adolescence in inducing several cognitive and behavioral alterations with possible relevance for neuropsychiatric disorders PMID: 24339333
  34. Data indicate Ca2+-dependent signaling pathways regulate AMPA-type glutamate receptor (AMPAR) GluA1 subunits phosphorylation at Thr-840 and Ser-845, and phosphorylation of one site inhibits phosphorylation of the other. PMID: 24706758
  35. THis study provides evidence that GluA1 mediated signaling is required for successful retention of spatial knowledge and that GluA1-containing AMPA receptors in hippocampus contribute to the process in a temporally modulated manner PMID: 23929622
  36. This study show that mice lacking GluA1-containing AMPA receptors are impaired at homing efficiently when allothetic cues are either unreliable or absent. We further demonstrate that they are unable to compute path integration coordinates. PMID: 24790195
  37. Data indicate that 6-chloro-4-ethyl-3,4-dihydro-2H-thieno[2,3-e]-1,2,4-thiadiazine 1,1-dioxide as the AMPA receptor potentiator. PMID: 24090223
  38. Spinal prostacyclin synthesis during early inflammation causes the recruitment of GluR1 receptors to membrane fractions PMID: 23969560
  39. Suppression of GluA1 expression by NaAsO2 seems at least partly responsible for neurite suppression induced by NaAsO2. PMID: 23694735
  40. The results of this study suggesting that the GluA1-dependent mechanisms only operate during the critical period. PMID: 24048851
  41. Data indicate that contextual discrimination involves term potentiation (LTP) promoted by calcium-permeable AMPA-type glutamate receptors, RAS-GRF1 and p38 MAP kinase. PMID: 23766509
  42. Genetic deletion of NP1 prevents hypoxic-ischemic neuronal death by reducing synaptic clustering of GluR1. PMID: 23525449
  43. There is an increase in GluA1 subunit exocytosis during long-term potentiation following disruption of the Rich2-Shank3 complex. PMID: 23739967
  44. Our results provide evidence for a role of hippocampal GluA1-containing AMPA receptors and their PDZ-interaction in experience-dependent expression of behavioral despair and link mechanisms of synaptic plasticity with behavioral expression of depression. PMID: 23262314
  45. hypoxic seizures in P9 C57BL/6N wild-type mice resulted in transient increases in GluR1 S831 and GluR1 S845 phosphorylation in cortex and were associated with enhanced seizure susceptibility to later-life kainic-acid-induced seizures PMID: 23223299
  46. RNF167 is a selective regulator of AMPAR-mediated neurotransmission. PMID: 23129617
  47. study suggests that the ROS-dependent changes in the phosphorylation and cell-surface localization of AMPA-Rs are necessary for dorsal horn neuron sensitization and thus, pain-related behavior PMID: 22770842
  48. the enhanced stress responsiveness in GluR-A(-/-) mice may be due, at least in part, to their inability to activate NMDA-mediated glutamatergic neurotransmission, suggesting that the integrity of AMPA/NMDA receptor function may be important for successful coping under stressful conditions. PMID: 20572199
  49. This study demonistrated that the fundamental role that GluA1-containing AMPARs play in activity-dependent increases in synaptic strength but do not support a GluA1-dependent mechanism for reductions in synaptic strength. PMID: 22197030
  50. GluA1 KO mice exhibited a context-dependent reduction in social behavior, showed faster acquisition and slower extinction of an instrumental stimulus-response, and displayed increased impulsive choice. PMID: 21693126

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic density membrane; Multi-pass membrane protein. Cell projection, dendrite. Cell projection, dendritic spine. Early endosome membrane; Multi-pass membrane protein. Recycling endosome membrane; Multi-pass membrane protein.
Protein Families
Glutamate-gated ion channel (TC 1.A.10.1) family, GRIA1 subfamily
Tissue Specificity
Expressed in the outer plexiform layer of the retina of the eye (at protein level).
Database Links
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