Recombinant Mouse Heme-binding protein 1 (Hebp1) comes from a mammalian cell expression system, which appears to support proper protein folding and post-translational modifications. The full-length protein spans amino acids 1 to 190 and includes a C-terminal hFc1 tag that helps with purification and detection. Purity levels reach above 85%, confirmed through SDS-PAGE analysis. This product is strictly for research purposes and cannot be used in clinical settings.
Heme-binding protein 1 (Hebp1) likely plays an important role in how cells handle heme metabolism. Scientists study this protein to understand its involvement in heme transport and regulation—processes that seem essential for keeping cells balanced and functioning properly. Learning how Hebp1 works may shed light on broader biochemical pathways related to heme use and could have implications across various biological contexts.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Antibody Development and Validation Studies
This recombinant mouse Hebp1 protein works well as an immunogen or standard when developing and testing antibodies against Hebp1. The C-terminal hFc1 tag makes purification and immobilization straightforward for ELISA-based antibody screening and validation work. Since it comes from a mammalian expression system, the protein maintains proper folding and modifications that appear crucial for creating antibodies that recognize native epitopes. The >85% purity should be adequate for immunization protocols and specificity testing.
2. Protein-Protein Interaction Studies
Researchers can use the hFc1-tagged Hebp1 in pull-down assays to find and study potential binding partners or interacting proteins from mouse tissue lysates or cell extracts. The Fc tag allows for efficient capture with Protein A/G beads, while the mammalian expression system likely preserves the native protein shape needed for normal interactions. Co-immunoprecipitation experiments can help confirm specific protein interactions discovered during initial screening.
3. Biochemical Characterization and Binding Assays
This recombinant protein offers a way to examine Hebp1's biochemical properties, including its reported ability to bind heme through in vitro binding studies. Having purified protein makes it possible to analyze binding kinetics, specificity, and stoichiometry using spectroscopic methods or other biochemical techniques. The mammalian expression system provides what appears to be properly folded protein that retains native binding characteristics—something that seems important for reliable biochemical studies.
4. ELISA-Based Detection and Quantification Assays
The hFc1-tagged Hebp1 can work as a standard or capture reagent in sandwich ELISA setups for detecting and measuring Hebp1 in biological samples. The Fc tag allows for oriented attachment to ELISA plates through anti-Fc antibodies, which may improve both assay sensitivity and consistency. This approach proves particularly valuable when studying Hebp1 expression levels across different mouse tissues or cell types in research settings.
