Recombinant Mouse Homeobox protein notochord (Noto)

Code CSB-YP722584MO
MSDS
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Source Yeast
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Code CSB-EP722584MO
MSDS
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Source E.coli
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Code CSB-EP722584MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP722584MO
MSDS
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Source Baculovirus
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Code CSB-MP722584MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Noto
Uniprot No.
Alternative Names
Noto; NotHomeobox protein notochord
Species
Mus musculus (Mouse)
Expression Region
1-240
Target Protein Sequence
MSSPAPSGTQ VQPGSLRPCP GAVSPVVPRR LARGRLESSF SVEAILARPK TRELAATSLP LSTCTSLNLL GAVSQYGVLP WVCSTGSWLP AYLSVGVYPL CSMSCVPGLN VTHHQQGLRL TGSELPYCLG PLKWAPTVDL RDHGTERHTK RVRTTFNLQQ LQELEKVFAK QHNLVGKERA QLAARLHLTE NQVRIWFQNR RVKYQKQQKL KLPSSSVMEE PSSSSDGNIQ SEDAELGIGS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that controls node morphogenesis. Acts downstream of both FOXA2 and Brachyury (T) during notochord development. Is essential for cilia formation in the posterior notochord (PNC) and for left-right patterning; acts upstream of FOXJ1 and RFX3 in this process and is required for the expression of various components important for axonemal assembly and function. Plays a role in regulating axial versus paraxial cell fate. Activates the transcription of ciliary proteins C11orf97 homolog, FAM183B and SPACA9 in the embryonic ventral node.
Gene References into Functions
  1. A novel mammal-specific three partite enhancer element regulates node and notochord-specific Noto expression. PMID: 23110100
  2. noto and foxj1 have roles in node formation, nodal ciliogenesis and cilia positioning PMID: 22357932
  3. findings suggest that Noto is involved in both rearrangement and morphogenesis of the tail bud during notochord formation. PMID: 20197654
  4. Noto ss an essential regulator of node morphogenesis and ciliogenesis in the posterior notochord. PMID: 17884984
  5. Lack of Noto correlates with misplacing and wrong directed differentiation of the tissue in the tail bud region PMID: 15550757

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Involvement in disease
Defects in Noto are the cause of the truncate (tc) phenotype. Truncate (tc) is a recessive mutation that affects the development of the caudal notochord. Mice homozygous for tc are viable but viability is reduced, which is attributed at least in part to spinal injury in the sacral and lower lumbar region.
Subcellular Location
Nucleus.
Database Links
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