Recombinant Mouse Insulinoma-associated protein 1 (Insm1)

Code CSB-YP734075MO
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Source Yeast
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Code CSB-EP734075MO
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Source E.coli
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Code CSB-EP734075MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP734075MO
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Source Baculovirus
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Code CSB-MP734075MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Insm1
Uniprot No.
Alternative Names
Insm1; Ia1; Insulinoma-associated protein 1; Zinc finger protein IA-1
Species
Mus musculus (Mouse)
Expression Region
1-521
Target Protein Sequence
MPRGFLVKRS KKSTPVSYRV RGGEDSDRAL LLSPGCGGAR AEPPVPSPGP LPPPPPPALA ERAHAALAAA LACAPGPPPP PPPGPRAAHF GNPEAAHPAP LYSPTRPVSR EHEKHKYFER SFNLGSPVSA ESFPTPAALL AGGGSGANGA GGGGGGTCGG DALLFAPAEL KMGTAFSAGA EAARGPGTGP PLSPAAALRP PGKRPAPPAA VATEPPAKAA KAPSAKKPKA IRKLHFEDEV TTSPVLGLKI KEGPVEAPRG RAGGATRPLG EFICQLCKEE YADPFALAQH KCSRIVRVEY RCPECAKVFS CPANLASHRR WHKPRPVPAA ARAPEPEAAT RAEAREAAGG GSSDRDTPSP GGVSESGSED GLYECHHCAK KFRRQAYLRK HLLAHHQALQ AKGAPPPPPP PPPPAEDILA FYAGPDEKAP QEASGDGEAA GVLGLSATAQ CHLCPVCGET FPSKGAQERH LRLLHAAQVF PCKYCPATFY SSPGLTRHIN KCHPSENRQV ILLQVPVRPA C
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Sequence-specific DNA-binding transcriptional regulator that plays a key role in neurogenesis and neuroendocrine cell differentiation during embryonic and/or fetal development. Binds to the consensus sequence 5'-[TG][TC][TC][TT][GA]GGG[CG]A-3' in target promoters. Acts as a transcriptional repressor of NEUROD1 and INS expression via its interaction with cyclin CCND1 in a cell cycle-independent manner. Negatively regulates skeletal muscle-specific gene expression in endocrine cells of the pituitary by inhibiting the Notch signaling pathway. Represses target gene transcription by recruiting chromatin-modifying factors, such as HDAC1, HDAC2, HDAC3, KDM1A and RCOR1 histone deacetylases. Binds to its own promoter, suggesting autoregulation as a self-control feedback mechanism. Competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4'. Promotes the generation and expansion of neuronal basal progenitor cells in the developing neocortex. Involved in the differentiation of endocrine cells of the developing anterior pituitary gland, of the pancreas and intestine, and of sympatho-adrenal cells in the peripheral nervous system. Promotes cell cycle signaling arrest and inhibition of cellular proliferation.
Gene References into Functions
  1. An INSM1/RCOR1/2 complex controls the balance of proliferation and differentiation during brain development. PMID: 28049845
  2. INSM1 ectopic expression impairs normal lung development especially in postnatal alveologenesis. PMID: 27072116
  3. Insm1 is the earliest known gene expressed in outer versus inner hair cells, demonstrating that nascent outer hair cells initiate a unique differentiation program in the embryo, much earlier than previously believed. PMID: 26545349
  4. Insm1-dependent Hes1 repression is required for neuroendocrine development. PMID: 26453796
  5. INSM1 interacts with RACK1 protein and facilitates insulin receptor -mediated signaling. PMID: 24407176
  6. Insm1 works to promote endocrine cell differentiation in a pathway that involves Neurog3 and Ripply3 PMID: 25053427
  7. Data suggest that IA-2 and IA-2beta play important roles in regulation of circadian rhythm, likely through neurochemical communication among neurons of suprachiasmatic nucleus. [Insm2-beta; IA-2beta; insulinoma-associated protein 2-beta] PMID: 22785238
  8. Insm1 acts in the epigenetic and transcriptional network that controls differentiation of endocrine cells in the anterior pituitary gland, and that it requires the SNAG domain to exert this function in vivo. PMID: 24227653
  9. This study suggested that Insm1 in promoting the transition of progenitors from apical and proliferative to basal, terminal and neuronogenic. PMID: 21284846
  10. isolation, characterization, and identification of an interacting protein, Cbl-associated protein (CAP) PMID: 12079283
  11. IA-1 gene is developmentally expressed in the nervous system and the NeuroD1/E47 transcription factors up-regulate IA-1 gene expression through the proximal E-box element of the IA-1 promoter PMID: 12890672
  12. Pancreatic and intestinal endocrine cells express Insm1 and require Insm1 for their development. PMID: 16951258
  13. The fetal lethality of Insm1 mutant mice is caused by catecholamine deficiency, which highlights the importance of Insm1 in the development of the sympatho-adrenal lineage. PMID: 18094025
  14. in mice and humans, INSM1 is likewise expressed transiently during terminal neurogenic divisions, from late progenitors to nascent neurons, and particularly during symmetric neuronogenic divisions. PMID: 18205207
  15. Insm1 and Nhlh1/NSCL1 are novel HH targets induced by Shh treatment in cultured cerebellar granule cell progenitors. PMID: 18231642
  16. INSM1 is a new zinc-finger transcription factor that modulates insulin gene transcription during early pancreas development. PMID: 18417529
  17. Insulinoma-associated 1 is expressed specifically in progenitors undergoing neurogenic divisions, has a panneurogenic role throughout the brain, and promotes basal progenitor formation in the neocortex. PMID: 18940587
  18. a conserved cis-regulatory sequence associated with Insm1 is bound by Ascl1 in the hindbrain, and Ascl1 is essential for the expression of Insm1 in the ventral hindbrain PMID: 19542360

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Subcellular Location
Nucleus.
Protein Families
INSM1 family
Tissue Specificity
Expressed in adrenal gland. Expressed in the dentate gyrus of the hippocampus and the wall of the lateral ventricle. Expressed in pancreatic and intestinal endocrine cells.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
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