Recombinant Mouse Integrator complex subunit 10(Ints10)

Code CSB-YP011758MO
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Source Yeast
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Code CSB-EP011758MO
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Source E.coli
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Code CSB-EP011758MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011758MO
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Source Baculovirus
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Code CSB-MP011758MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Ints10
Uniprot No. Q8K2A7
Alternative Names Ints10; Integrator complex subunit 10; Int10
Species Mus musculus (Mouse)
Expression Region 1-710
Target Protein Sequence MSAQGDCEFL VQRARELVPQ DLWAAKAWLI TARSLYPADF NIQYEMYTIE RNAERTATAG RLLYDMFVNF PDQPVVWREI SIITSALRND SQDKQTQFLR SLFETLPGRV QCEMLLKVTE QCFNTLERSE MLLLLLRRFP ETVVQHGVGL GEALLEAETI EEQDSPVNCF RKLFVCDVLP LIINNHDVRL PANLLYKYLN KAAEFYINYV TRSTQSENQH QGAQDTSDLM SPSKRSSQKY IIEGLTEKSS HIVDPWERLF KILNVVGMRC EWQMDKGRRS CSDLLHRMKE LCRYMNSFDS EAHNNYKNQV LYSTMLVFFK SAFQYVSSIQ PSLFQGPNAP SQVPLILLED VANVYGDVEI DRSKHIHKKR KLAEGREKTM SSDDEECSAK GRNRHIVVSK ADLSNSIEVL ESFKLARESW ELLYSLEFLD KEFTRICLAW KTDTWLWLRI FLTDMIIYQG QYKKAIASLH HLAALQGSLS QPQITGQGTL EHQRALIQLA TCHFALGEYR MTCEKVLDLM CYMVLPIQDG GKPQEEPSKV KPKCRKGLDL KLLPCTSKAI MPYCLHLMLA CFKLRAFTDS RDDMALGHVI VLLQQEWPRG ENLFLKAISK ICQQGNFQYE NFFSYVTNID MLEEFAYLRT QEGGKIHLEL LPNQGMLIKH HTVTRGITKG VKEDFRLAME RQVSRCGENL MAVLHRFCIN EKILLLQTLT
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. May be not involved in the recruitment of cytoplasmic dynein to the nuclear envelope by different components of the INT complex.
Subcellular Location Nucleus.
Protein Families Integrator subunit 10 family
Database Links

KEGG: mmu:70885

STRING: 10090.ENSMUSP00000034328

UniGene: Mm.252421

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