Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Itgb1

Uniprot No.

P09055

Research Area

Signal Transduction

Alternative Names

Itgb1; Integrin beta-1; Fibronectin receptor subunit beta; VLA-4 subunit beta; CD antigen CD29

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

21-728aa

Target Protein Sequence

QTDKNRCLKANAKSCGECIQAGPNCGWCTNTTFLQEGMPTSARCDDLEALKKKGCQPSDIENPRGSQTIKKNKNVTNRSKGMAEKLRPEDITQIQPQQLLLKLRSGEPQKFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTDRGEFFNELVGQQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNVYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSGNSSNVIQLIIDAYNSLSSEVILENSKLPDGVTINYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITANKCPNKESETIKIKPLGFTEEVEVVLQFICKCNCQSHGIPASPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCRCRVCECYPNYTGSACDCSLDTGPCLASNGQICNGRGICECGACKCTDPKFQGPTCETCQTCLGVCAEHKECVQCRAFNKGEKKDTCAQECSHFNLTKVESREKLPQPVQVDPVTHCKEKDIDDCWFYFTYSVNGNNEAIVHVVETPDCPTGPD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

82.2kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This recombinant Mouse Itgb1 protein is an E.coli expressed mature Extracellular Domain protein with N-terminal 6xHis tag and its purity is 90%+ determined by SDS-PAGE. With the appropriate cDNA and PCR methods, Itgb1 expression plasmids can be rapidly produced. which must undergo denaturation and folding cycle, can be recovered with more modest yields. Hence, using small-scale fermentations and laboratory-scale processing equipment, Itgb1 proteins (or subdomains thereof) can usually be produced in sufficient quantities to initiate most studies including detailed structural determinations.which must undergo denaturation and folding cycle, can be recovered with more modest yields. Hence, using small-scale fermentations and laboratory-scale processing equipment, Itgb1 proteins (or subdomains thereof) can usually be produced in sufficient quantities to initiate most studies including detailed structural determinations.

Itgb1 is involved in cell adhesion, migration, and proliferation, among other cellular functions. Itgb1 is overexpressed in cancer cells, contributing to epithelial-mesenchymal transition (EMT) and tumor metastasis, among other malignant characteristics. It has been discovered that Itgb1 promotes gastric cancer cell proliferation, survival, and motility. Through negative regulation of the Notch pathway, Itgb1 can greatly enhance glioma cell proliferation. Itgb1 is a key player in the formation of vasculogenic mimicry (VM) in human cancer cells, suggesting suppression of Itgb1 could be a promising new treatment option for cancer.

Target Background

Function

Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion. Integrin alpha-4/beta-1 is a receptor for VCAM1 and recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling.; Isoform 2 displaces isoform 1 in striated muscles.

Gene References into Functions

These findings suggest that integrin beta1 may be an oxygen-sensitive molecule that promotes keratinocyte migration during wound healing and that Notch1 signaling is involved in this process. PMID: 28266574
During differentiation of adult-born dentate gyrus granule cells, Sema7A promotes dendrite growth, complexity and spine development through beta1-subunit-containing integrin receptors. PMID: 28281529
that Mzb1 (-/-) plasmablasts show a reduced activation of beta1-integrin, which contributes to the impaired plasmablast differentiation and migration of antibody-secreting cells to the bone marrow. PMID: 30257949
beta1-integrin is a key mediator of the Numb pathway in cardiac progenitor cell maintenance. PMID: 29653101
beta1-integrin promotes endothelial barrier disruption during inflammation, and targeting beta1-integrin signaling could serve as a novel means of blocking pathological vascular leak. PMID: 29941602
Serine/threonine-protein kinase proteins, also known as P21-activated kinase (PAK), and the mechanosensitive factor, Yes-associated protein 1 (YAP-1) are core mediators of pro-fibrotic integrin beta-1 signaling. PMID: 27535340
the effect of NCAM on tyrosine phosphorylation of beta1 integrin was independent of the fibroblast growth factor receptor. These results indicated that NCAM regulates directional lamellipodia formation of BMSCs through beta1 integrin signal-mediated cofilin activity. PMID: 28536952
study of the interplay between the EDN3 and beta1-integrin signalling pathways during ENS ontogenesis and the role of EDN3 in enteric neural crest cell adhesion PMID: 27905407
Beta 1 integrin silencing was able to downregulate the transcription of storeoperated Ca2+ entry associated genes to normal levels, including calcium releaseactivated calcium modulator 1 and short transient receptor potential channel member 1, but not stromal interaction molecule 1, in asthma. PMID: 28656279
epithelial integrin beta1 functions as a critical and canonical regulator of developmental angiogenesis in the pituitary gland. PMID: 27810956
These results indicate a developmentally regulated function of alpha5beta1 integrin in microglial migration during colonization of the embryonic brain. PMID: 28417486
Dissociated murine embryonic lung cells self-assemble into three-dimensional pulmonary bodies that are dependent on alpha5beta1 integrin mediated fibrillogenesis for cell-cell mediated self-assembly: compaction and epithelial cyst formation. PMID: 28370666
Irradiation coupled with JNK inhibition in beta1 integrin -/- transgenic adenocarcinoma of prostate (TRAMP) leads to increased levels of nuclear focal adhesion kinase (FAK) in tumor cells. PMID: 27438371
Integrin beta1 and dystroglycan compensate each other to mediate laminin-dependent basement membrane assembly and epiblast polarization. PMID: 27449702
The results of this study suggest that the effects of beta1-integrin gene knockout on these results suggest that the effects of beta1-integrin gene knockout on NSC maintenance and fate commitment in the DG follow a substantially slower time course in males compared to females because of increased persistence of beta1-integrin protein in males. PMID: 27145730
the cross-talk between SFRP4, integrin alpha1beta1, and Notch1 suppresses the cardiac differentiation of P19CL6 cells. PMID: 27542621
talins are essential for kidney collecting duct development through mechanisms that extend beyond those requiring binding to the beta1 integrin subunit NPxY motif. PMID: 28993400
Mechanistically, ligation of alpha8beta1 by the milk protein Mfge8 reduces antral smooth muscle contractile force by preventing RhoA activation through a PTEN-dependent mechanism. PMID: 27092791
Itgb1 inhibition alleviates the chronic hyperproliferative dermatitis phenotype of Sharpin-deficient mice. PMID: 29040328
Expression of beta1-integrin in collecting duct principal cellss is a critical determinant of renal structural and functional integrity. PMID: 28701310
Taken together, acid-induced acute lung injury results in epithelial microvesicle shuttling of miR-17/221 that in turn modulates macrophage beta1 integrin recycling, promoting macrophage recruitment and ultimately contributing to lung inflammation. PMID: 28674181
Type I collagen was highly expressed in the spinal cord during the scar-forming phase and induced astrocytic scar formation via the integrin-N-cadherin pathway. PMID: 28628111
the transcription regulators YAP and TAZ localise to the nucleus in the basal layer of skin and are elevated upon wound healing. PMID: 26989177
Itgb1 is an essential niche molecule that maintains satellite cell (SC) homeostasis, and sustains the expansion and self-renewal of this stem cell pool during regeneration. We further show that Itgb1 cooperates with fibroblast growth factor 2 (Fgf2), a potent growth factor for SCs, to synergistically activate their common downstream effectors, the mitogen-activated protein (MAP) kinase Erk and protein kinase B (Akt). PMID: 27376575
b1-integrin has an important role in fine-tuning the response of the early lens to the gradient of growth factors that regulate lens fiber cell differentiation. PMID: 27596755
results demonstrate that c-Cbl mediates the ubiquitination/degradation of integrin beta1, which leads to COMP deficiency-induced dilated cardiomyopathy. PMID: 27693578
Data show that beta1 integrin facilitate blood vessel-guided neuronal migration in injured brain. PMID: 28153772
By modulating its affinity with kindlin, beta3 integrin may be able to locate near the cell edge where it can control beta1 integrin activation and clustering. PMID: 27990663
These findings indicated that mechanical strain promoted osteoblastic differentiation through integrinbeta1mediated beta-catenin signaling. PMID: 27314666
Staphylococcus aureus induces up-regulation of beta1 integrin in mast cells. PMID: 26595647
TERT may promote gastric cancer metastasis through the TERT-miR-29a-ITGB1 regulatory pathway. PMID: 26903137
the rate of collagen I degradation was increased in Poldip2(+/-) vs. Poldip2(+/+) MASMs. Conversely, activation of the PI3K/Akt/mTOR signaling pathway, involved in regulation of protein synthesis, was significantly elevated in Poldip2(+/-) MASMs as was beta1-integrin expression. PMID: 26801741
These findings show that talin and kindlin cooperatively activate integrins leading to fibronectin binding and adhesion. PMID: 26821125
Integrin alpha11beta1, a receptor for fibrillar collagen, plays a role in differentiation of fibroblasts into carcinoma-associated fibroblasts in NSCLC xenografts. PMID: 26148229
High glucose decreases the expression of integrin beta1 in cultured podocytes, accompanied with an increase of integrin beta3. PMID: 26352002
LRP1 interacts with beta1-integrin mediating integrin internalization and thus correlates with downstream signaling of beta1-integrin such as focal adhesion dynamics PMID: 26610862
4.1G has a role in cell adhesion, spreading, and migration in MEF cells by modulating the surface expression of beta1 integrin and subsequent downstream signal transduction PMID: 26644476
DPP-4 and integrin beta1 interactions regulate key endothelial cell signal transduction in both physiological and pathological conditions including endothelial mesenchymal transformation. PMID: 25830763
establish a paradigm according to which retrograde trafficking, and not endosomal recycling, is the key driver for beta1 integrin function in highly polarized cells PMID: 26641717
RECK-mediated beta1-Integrin regulation by TGF-beta1 is critical for wound contraction in mice. PMID: 26247610
Intravenous administration of endothelial colony-forming cells overexpressing integrin beta1 augments angiogenesis in ischemic legs. PMID: 26702126
The findings of this study showed that astrocytic beta1-itg has an important role in defining cellular properties of the blood brain barrier in the cerebral cortex. PMID: 25997909
the epithelial interaction between fibronectin and beta1 integrin is important for ameloblast differentiation and enamel formation. PMID: 25830530
Data show that active active focal adhesion kinase (FAK) localizes to integrin-containing endosomes. PMID: 26436690
Integrin beta1 controls VE-cadherin localization, has critical functions in the growing and maturing vasculature, and is required for the formation of stable, non-leaky blood vessels. PMID: 25752958
Data identifies integrin beta1 as a part of the osmosensing machinery and regulator of cisplatin resistance in adherent Ehrlich cells. PMID: 25925201
Mitochondrial oxidative phosphorylation regulates integrin-beta1 modifications and enhances cell motility. PMID: 25504047
C1GALT1 promotes invasive phenotypes of hepatocellular carcinoma cells by modulating integrin beta1 glycosylation and activity. PMID: 25089569
caveolin-dependent endocytosis was discovered to be involved in the regulation of the enrichment of beta1 integrin on the cell surface after stimulation by hypergravity PMID: 26071356
We propose that neuronal morphology may be directed during development and regeneration by factors that influence how beta1-integrin, FAK and RhoGTPase molecules integrate substrate signals in the 3D microenvironment. PMID: 23764511

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell projection, invadopodium membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Recycling endosome. Melanosome. Cell projection, lamellipodium. Cell projection, ruffle. Cell junction, focal adhesion. Cell surface.; [Isoform 2]: Cell membrane, sarcolemma. Cell junction. Note=In cardiac muscle, isoform 2 is found in costameres and intercalated disks.

Protein Families

Integrin beta chain family

Tissue Specificity

Isoform 2 is expressed in skeletal and cardiac muscles only (at protein level). Isoform 1 is very weakly expressed in striated muscles and not detected in adult skeletal muscle fibers and cardiomyocytes.

Database Links

KEGG: mmu:16412

STRING: 10090.ENSMUSP00000087457

UniGene: Mm.263396

Code	CSB-EP011880MO
Abbreviation	Recombinant Mouse Itgb1 protein, partial
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Integrin beta-1 (Itgb1), partial

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