Recombinant Mouse Krueppel-like factor 3 (Klf3)

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

Klf3

Uniprot No.

Q60980

Alternative Names

Klf3; Bklf; Krueppel-like factor 3; Basic krueppel-like factor; CACCC-box-binding protein BKLF; TEF-2

Species

Mus musculus (Mouse)

Expression Region

1-344

Target Protein Sequence

MLMFDPVPVK QEAMDPVSVS FPSNYIESMK PNKYGVIYST PLPDKFFQTP EGLTHGIQVE PVDLTVNKRG SPPAAGGSPS SLKFPSHRRA SPGLSMPSSS PPIKKYSPPS PGVQPFGVPL SMPPVMAAAL SRHGIRSPGI LPVIQPVVVQ PVPFMYTSHL QQPLMVSLSE EMDNSNSGMP VPVIESYEKP LLQKKIKIEP GIEPQRTDYY PEEMSPPLMN PVSPPQALLQ ENHPSVIVQP GKRPLPVESP DTQRKRRIHR CDYDGCNKVY TKSSHLKAHR RTHTGEKPYK CTWEGCTWKF ARSDELTRHF RKHTGIKPFQ CPDCDRSFSR SDHLALHRKR HMLV

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis.

Gene References into Functions

mechanistic insights into the regulation of Lgals3, demonstrating that C-terminal binding protein (CtBP) is required to drive optimal KLF3-mediated silencing. PMID: 27226561
Phosphorylation of KLF3 and CtBP2 by HIPK2 strengthens the interaction between these two factors and increases transcriptional repression by KLF3. PMID: 25659434
We conclude that KLF3 plays diverse and important roles in cardiovascular development and function in mice. PMID: 23874215
KLF3 binds the Fam132a promoter in vitro and in vivo and that this leads to repression of promoter activity. Fam132a is significantly upregulated in the absence of KLF3. PMID: 23633521
KLF3 and KLF8 have overlapping roles in vivo and participate in the silencing of embryonic globin expression during development. PMID: 23716600
we concluded that KLF3 is a transcriptional repressor for wee1 gene. PMID: 22115574
KLF3 is a driving force toward marginal zone B-cell maturation. PMID: 21297003
KLF3 is an important regulator of several biological processes, including adipogenesis, erythropoiesis, and B cell development. (Review) PMID: 21360637
role for KLF3 in muscle gene regulation and an alternate mechanism for transcriptional regulation by SRF via its recruitment to KLF binding sites PMID: 20404088
Proviral integration of an Abelson-murine leukemia virus deregulates BKLF-expression in the hypermutating pre-B cell line 18-81. PMID: 15829312
basic Kruppel-like factor gene (Klf3) has a role with CtBP in adipogenesis PMID: 18391014

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Subcellular Location

Nucleus.

Protein Families

Krueppel C2H2-type zinc-finger protein family

Tissue Specificity

In 8.5 day embryos, expressed in midbrain, anterior hindbrain and ventral forebrain. In 9 day embryos, expressed throughout ventral anterior half of embryo including midbrain-hindbrain junction, ventral midbrain, diencephalon and forebrain. At 10.5 days,

Database Links

KEGG: mmu:16599

STRING: 10090.ENSMUSP00000128429

UniGene: Mm.392759

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Recombinant Mouse Krueppel-like factor 3 (Klf3)

Product Details

Customer Reviews and Q&A

Target Background

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