Recombinant Mouse Leucine-rich repeat protein SHOC-2 (Shoc2)

Code CSB-YP021275MO
MSDS
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Source Yeast
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Code CSB-EP021275MO
MSDS
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Source E.coli
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Code CSB-EP021275MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021275MO
MSDS
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Source Baculovirus
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Code CSB-MP021275MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Shoc2
Uniprot No.
Alternative Names
Shoc2; Leucine-rich repeat protein SHOC-2; Protein soc-2 homolog; Protein sur-8 homolog
Species
Mus musculus (Mouse)
Expression Region
1-582
Target Protein Sequence
MSSSLGKEKD SKEKDPKVPS AKEREKESKA SGGFGKESKE KEPKAKGKDA KDGKKESSAA QPGVAFSVDN TIKRPNPAPG TRKKSSNAEV IKELNKCREE NSMRLDLSKR SIHILPPSVK ELTQLTELYL YSNKLQSLPA EVGCLVNLMT LALSENSLTS LPDSLDNLKK LRMLDLRHNK LREIPSVVYR LDSLTTLYLR FNRITTVEKD IKNLPKLSML SIRENKIKQL PAEIGELCNL ITLDVAHNQL EHLPKEIGNC TQITNLDLQH NDLLDLPDTI GNLSSLNRLG LRYNRLSAIP RSLAKCSALE ELNLENNNIS TLPESLLSSL VKLNSLTLAR NCFQLYPVGG PSQFSTIYSL NMEHNRINKI PFGIFSRAKV LSKLNMKDNQ LTSLPLDFGT WTSMVELNLA TNQLTKIPED VSGLVSLEVL ILSNNLLKKL PHGLGNLRKL RELDLEENKL ESLPNEIAYL KDLQKLVLTN NQLSTLPRGI GHLTNLTHLG LGENLLTHLP EEIGTLENLE ELYLNDNPNL HSLPFELALC SKLSIMSIEN CPLSHLPPQI VAGGPSFIIQ FLKMQGPYRA MV
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and participates in MAPK pathway activation. Upon M-Ras/MRAS activation, targets PP1c to specifically dephosphorylate the 'Ser-259' inhibitory site of RAF1 kinase and stimulate RAF1 activity at specialized signaling complexes.
Gene References into Functions
  1. Results provide evidence that SHOC2 trapping at different subcellular sites has a diverse impact on ERK signaling strength and dynamics, suggesting a dual counteracting modulatory role of SHOC2 in the control of ERK signaling exerted at different intracellular compartments. PMID: 27466182
  2. Data suggest that SUR-8 may act in an ERK-independent pathway to regulate AVC development. PMID: 20549726
  3. Erbin has a regulatory role in the Ras-Raf-MEK pathway and may inhibit ERK activation by disrupting the Sur-8-Ras/Raf interaction PMID: 16301319
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
SHOC2 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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