Product Details

Purity

>85% (SDS-PAGE)

Target Names

Ldlrap1

Uniprot No.

Q8C142

Alternative Names

Ldlrap1; ArhLow density lipoprotein receptor adapter protein 1; Autosomal recessive hypercholesterolemia protein homolog

Species

Mus musculus (Mouse)

Expression Region

1-308

Target Protein Sequence

MDALKSAGRA LIRSPSLAKQ SWAGGRHRKL PENWTDTRET LLEGMVFSLK YLGMTLVERP KGEELSAAAV KRIVATAKAS GKKLQKVTLK VSPRGIILTD SLTSQLIENV SIYRISYCTA DKMHDKVFAY IAQSQQNESL ECHAFLCTKR KVAQAVTLTV AQAFKVAFEF WQVSKEEKEK REKANQEGGD VPGTRRDSTP SLKTLVATGN LLDLEEVAKA PLSTVSANTN NVDETPRPQV LGNNSVVWEL DDGLDEAFSR LAQSRTNPQV LDTGLSAQDI HYAQCLSPTD WDKPDSSGID QDDDVFTF

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. Required for trafficking of LRP2 to the endocytic recycling compartment which is necessary for LRP2 proteolysis, releasing a tail fragment which translocates to the nucleus and mediates transcriptional repression.

Gene References into Functions

The combination of Arh and Dab2 is responsible for the majority of adaptor function in LDLR endocytosis and LDLR-mediated cholesterol homeostasis. PMID: 27005486
cells that depend upon ARH for LDL uptake can control which lipoproteins are internalized by their LDLRs through changes in nitric oxide. PMID: 23564733
results are consistent with LDL receptor adaptor protein(ARH) playing a critical and specific role in low density lipoprotein receptor endocytosis in the liver PMID: 12746448
Hepatocytes do not take-up LDL in vivo without ARH protein, but they normally catabolize LDL in vitro. Thus, the requirement of ARH protein for proper functioning of the LDLR is not cell-specific, but rather may depend on the cellular environment. PMID: 15472122
the LDL receptor alone can account for the clearance of apoE-containing lipoproteins in mice, and the contribution of other receptors is minimal, and b) defects in either increase the sensitivity to apoE-induced hypertriglyceridemia in mice. PMID: 16339113
Taken together, our data suggest that ARH is a multifunctional protein whose spectrum of function in the brain goes beyond the traditionally known metabolism of lipoproteins, and that ARH may be locally synthesized in the axon. PMID: 17727637
the endocytic adaptor protein ARH associates with motor and centrosomal proteins and is involved in centrosome assembly and cytokinesis PMID: 18417616
Loss of gamma-secretase function decreased endocytosis of low-density lipoprotein (LDL) receptor PMID: 19005074
ARH marks ROMK for clathrin-dependent endocytosis, in concert with the demands of potassium homeostasis PMID: 19841541

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Subcellular Location

Cytoplasm.

Database Links

KEGG: mmu:100017

STRING: 10090.ENSMUSP00000036749

UniGene: Mm.27486

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Recombinant Mouse Low density lipoprotein receptor adapter protein 1 (Ldlrap1)

Product Details

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