Recombinant Mouse Lysyl oxidase homolog 2(Loxl2)

Code CSB-EP013041MO
Size US$2466
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP013041MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Loxl2.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP013041MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Loxl2.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Loxl2
Uniprot No. P58022
Research Area Others
Alternative Names Loxl2Lysyl oxidase homolog 2; EC 1.4.3.13; Lysyl oxidase-like protein 2
Species Mus musculus (Mouse)
Source E.coli
Expression Region 26-776aa
Target Protein Sequence QYEGWPYQLQYPEYFQQPAPEHHQRQVPSDVVKIQVRLAGQKRKHNEGRVEVYYEGQWGTVCDDDFSIHAAHVVCRQVGYVEAKSWAASSSYGPGEGPIWLDNIYCTGKESTLASCSSNGWGVTDCKHTEDVGVVCSEKRIPGFKFDNSLINQIESLNIQVEDIRIRPILSAFRHRKPVTEGYVEVKEGKAWKQICNKHWTAKNSHVVCGMFGFPAEKTYNPKAYKTFASRRKLRYWKFSMNCTGTEAHISSCKLGPSVTRDPVKNATCENGQPAVVSCVPSQIFSPDGPSRFRKAYKPEQPLVRLRGGAQVGEGRVEVLKNGEWGTICDDKWDLVSASVVCRELGFGTAKEAITGSRLGQGIGPIHLNEVQCTGTEKSIIDCKFNTESQGCNHEEDAGVRCNIPIMGFQKKVRLNGGRNPYEGRVEVLTERNGSLVWGTVCGQNWGIVEAMVVCRQLGLGFASNAFQETWYWHGNIFANNVVMSGVKCSGTELSLAHCRHDEEVACPEGGVRFGAGVACSETAPDLVLNAEIVQQTAYLEDRPMSLLQCAMEENCLSASAVHTDPTRGHRRLLRFSSQIHNNGQSDFRPKNGRHAWIWHDCHRHYHSMEVFTYYDLLSLNGTKVAEGHKASFCLEDTECEGDIQKSYECANFGEQGITMGCWDMYRHDIDCQWIDITDVPPGDYLFQVVINPNYEVPESDFSNNIMKCRSRYDGYRIWMYNCHVGGAFSEETEQKFEHFSGLLNNQLSVQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 88.4kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E-cadherin, probably by mediating deamination of histone H3 (By similarity). Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation.
Gene References into Functions
  1. although Loxl2 is expressed in both dermis and epidermis, its function appears largely confined to the epidermis. PMID: 29953488
  2. LOXL2 controls myofibroblast transformation and migration. PMID: 27966531
  3. The mRNA and protein expression levels of LOXL2 were higher in a mouse model of tubulointerstitial fibrosis compared with in control mice. PMID: 28677767
  4. Findings indicate a pathophysiologic role and function for lysyl oxidase-like protein LOXL2 in breast cancer metastasis. PMID: 28720577
  5. Insulin resistance promotes lysyl oxidase like 2 induction and fibrosis accumulation in non-alcoholic fatty liver disease. PMID: 28468951
  6. glomerular extracellular matrix. Altogether, we demonstrate that LOXL2 is a novel component of the molecular machinery that forms cross-linked collagen IV networks, which are essential for glomerular basement membrane stability and molecular ultrafiltration function. PMID: 27770022
  7. LOX and LOXL2 may play an important role in the pathogenesis of AMD. Targeting LOXL2 could have a broader efficacy than targeting LOX, by reducing angiogenesis and inflammation, as well as fibrosis. PMID: 26258612
  8. Loss and gain of function analyses combined with in vivo studies in syngeneic breast cancer models demonstrate the participation of LOXL2 and E47 in tumor growth and their requirement for lung metastasis. PMID: 24632622
  9. promoted intrahepatic metastasis by increasing tissue stiffness PMID: 25048396
  10. Findings reveal new insight into the mechanisms of fibroblast activation, a novel function of LOXL2, and further highlight the importance of generating LOXL2-targeted therapies for the prevention of tumor progression and metastasis. PMID: 24008674
  11. The Snail1 transcription factor represses mouse pericentromeric transcription, acting through the H3K4 deaminase LOXL2. PMID: 24239292
  12. The enzymatic activity of lysyl oxidas-like-2 (LOXL2) is not required for LOXL2-induced inhibition of keratinocyte differentiation. PMID: 22157764
  13. This study provides the first evidence for the role of LOXL2 in regulating angiogenesis through collagen IV scaffolding. PMID: 21835952
  14. LOXL2 promotes chondrocyte differentiation by mechanisms that are likely to include roles as both a regulator and an effector of chondrocyte differentiation PMID: 21071451
  15. The efficacy and safety of LOXL2-specific monoclonal antibody represents a new therapeutic approach with broad applicability in oncologic and fibrotic diseases. PMID: 20818376
  16. LoxL2 is not expressed in MC3T3-E1 cells. PMID: 15652501

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Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane, Nucleus, Chromosome
Protein Families Lysyl oxidase family
Tissue Specificity Ubiquitous. Highest expression in skin, lung and thymus. Present in chondrocytes: mainly expressed by chondrocytes in healing fractures and in epiphyseal growth plates (at protein level).
Database Links

KEGG: mmu:94352

STRING: 10090.ENSMUSP00000022660

UniGene: Mm.116714

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