Recombinant Mouse M-phase inducer phosphatase 2 (Cdc25b)

Code CSB-YP004995MO
MSDS
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Source Yeast
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Code CSB-EP004995MO
MSDS
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Source E.coli
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Code CSB-EP004995MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004995MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP004995MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cdc25b
Uniprot No.
Alternative Names
Cdc25b; Cdc25m2; M-phase inducer phosphatase 2; EC 3.1.3.48; Dual specificity phosphatase Cdc25B
Species
Mus musculus (Mouse)
Expression Region
1-576
Target Protein Sequence
MEVPLQKSAP GSALSPARVL GGIQRPRHLS VFEFESDGFL GSPEPTASSS PVTTLTQTMH NLAGLGSEPP KAQVGSLSFQ NRLADLSLSR RTSECSLSSE SSESSDAGLC MDSPSPVDPQ MAERTFEQAI QAASRVIQNE QFTIKRFRSL PVRLLEHSPV LQSITNSRAL DSWRKTEAGY RAAANSPGED KENDGYIFKM PQELPHSSSA QALAEWVSRR QAFTQRPSSA PDLMCLTTEW KMEVEELSPV AQSSSLTPVE RASEEDDGFV DILESDLKDD EKVPAGMENL ISAPLVKKLD KEEEQDLIMF SKCQRLFRSP SMPCSVIRPI LKRLERPQDR DVPVQSKRRK SVTPLEEQQL EEPKARVFRS KSLCHEIENI LDSDHRGLIG DYSKAFLLQT VDGKHQDLKY ISPETMVALL TGKFSNIVEK FVIVDCRYPY EYEGGHIKNA VNLPLERDAE TFLLQRPIMP CSLDKRIILI FHCEFSSERG PRMCRFIRER DRAANDYPSL YYPEMYILKG GYKEFFPQHP NFCEPQDYRP MNHEAFRDEL RNFRLKTRSW AGERSRRELC SRLQDQ
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity.
Gene References into Functions
  1. data suggest an important role of CDC25B for microtubule nucleation and organization. N-terminal of CDC25B is required for regulating the microtubule dynamics and mitotic function. PMID: 27885657
  2. LSD1 is essential for oocyte meiotic progression by upregulating CDC25B expression. PMID: 26626423
  3. The role of Cdc25c and Cdc25b in activating G2/M cell cycle checkpoint in zygote. PMID: 25012723
  4. AURKA induced phosphorylation and recruitment of CDC25B to MTOCs prior to p-Cyclin B1-Ser123, and this sequential regulation is essential for the commitment of the oocytes to resume meiosis. PMID: 25349079
  5. Data indicate that 14-3-3epsilon is required for the mitotic entry in the fertilized mouse eggs and responsible for sequestering the CDC25B in cytoplasm. Its binding to CDC25B-S321 phosphorylated by PKA induces mitotic arrest. PMID: 25059436
  6. contributes to metaphase arrest in mouse oocytes PMID: 23661366
  7. Ser321 of Cdc25B is the specific binding site for 14-3-3epsilon binding. PMID: 23326474
  8. Protein kinase A the early development of mouse embryos by phosphorylation of S149 and S321 of CDC25B, which plays an important role in the regulation of G(2)/M transition in the mitotic cell cycle of fertilized mouse eggs. PMID: 22348958
  9. Cdc25B overexpression in early mouse two-cell embryos reverses two-cell block and promotes their development into four-cell stage by activating MPF. PMID: 21141569
  10. MCPH1, through its function in the Chk1-Cdc25-Cdk1 pathway to couple the centrosome cycle with mitosis, is required for precise mitotic spindle orientation and thereby regulates the progenitor division mode to maintain brain size. PMID: 21947081
  11. Ser(149) may be another potential PKA phosphorylation target of Cdc25B in G(2)/M transition of fertilized mouse eggs and Cdc25B as a direct downstream substrate of PKA in mammals PMID: 21212267
  12. Cdc25b phosphatase is required for resumption of meiosis during oocyte maturation PMID: 11912493
  13. Cdc25A, or possibly other phosphatases, is able to functionally compensate for the loss of Cdc25B and Cdc25C in mice PMID: 15767688
  14. Shh contributes to CDC25B transcriptional activation in the neural tube both of chick and mouse embryos PMID: 16564519
  15. Cdc25b is regulated by Foxm1 during heart development. PMID: 17366632
  16. p38 regulates the timing of mitotic entry via modulation of Cdc25B activity under normal nonstress conditions PMID: 17548358
  17. Data show that the expression of cell cycle genes, Cdc25b, in the optic nerves is downregulated in a conditional ablation of Shh mutant mice. PMID: 18625210
  18. On the first mitotic division of mouse fertilized eggs, PRKACA regulates G2/M transition by phosphorylating CDC25B-Ser321 but not CDC25B-Ser229. PMID: 18633139
  19. Cdc25B subcellular localization determines its function during the process of maintaining germinal vesicle arrest in mouse oocytes PMID: 19035343
  20. Results suggest that Cdc25B is a direct target of PKA in prophase-arrested oocytes and that Cdc25B phosphorylation results in its inhibition and sequestration by the 14-3-3 protein. PMID: 19223768

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Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
Protein Families
MPI phosphatase family
Tissue Specificity
Expressed predominantly in spleen, lung, heart, brain, intestine, and muscle.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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