Recombinant Mouse Matrix metalloproteinase-20 (Mmp20)

Code CSB-EP014667MO
MSDS
Size $306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Target Names
Mmp20
Uniprot No.
Research Area
Cancer
Alternative Names
MMP-20;Enamel metalloproteinase;Enamelysin
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
107-482aa
Target Protein Sequence
YRLFPGEPKWKKNILTYRISKYTPSMSPTEVDKAIQMALHAWSTAVPLNFVRINSGEADIMISFETGDHGDSYPFDGPRGTLAHAFAPGEGLGGDTHFDNAEKWTMGTNGFNLFTVAAHEFGHALGLGHSTDPSALMYPTYKYQNPYRFHLPKDDVKGIQALYGPRKIFPGKPTMPHIPPHKPSIPDLCDSSSSFDAVTMLGKELLFFKDRIFWRRQVHLPTGIRPSTITSSFPQLMSNVDAAYEVAERGIAFFFKGPHYWVTRGFHMQGPPRTIYDFGFPRHVQRIDAAVYLKEPQKTLFFVGEEYYSYDERKKKMEKDYPKNTEEEFSGVSGHIDAAVELNGYIYFFSGRKTFKYDTEKEDVVSVVKSSSWIGC
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
49.7 kDa
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. Cleaves aggrecan at the '360-Asn-|-Phe-361' site.
Gene References into Functions
  1. increased cadherin cleavage by transgenic MMP20 in the wild type background releases excess beta-catenin, which translocates to ameloblast nuclei to promote cell migration/invasion. Therefore, MMP20 plays a role in normal ameloblast migration through tightly controlled Wnt signaling and MMP20 overexpression disrupts this process. PMID: 27403713
  2. MMP-20 action guides the growth morphology of the forming hydroxyapatite crystals and enhances their crystallinity. PMID: 26513418
  3. Matrix metalloproteinase-20 over-expression is detrimental to enamel development PMID: 24466234
  4. MMP20 cleaves extracellular domains of cadherin adherens junction proteins, both E- and N-cadherin transcripts are expressed at significantly higher levels in Mmp20 null mice; in Mmp20 ablated mice N-cadherin expression persists during maturation stage PMID: 24067343
  5. enamel of M180Tg/AmelxKO mice was thinner than WT, it had similar mechanical properties and decussating enamel prisms, which were abolished by the loss of MMP20 in the M180Tg/DKO mice. PMID: 24072097
  6. required for ameloblast cell movement necessary to form the decussating enamel rod patterns, for the prevention of ectopic mineral formation, and to maintain a functional dentin-enamel junction PMID: 22243247
  7. MMP20 may influence ameloblast developmental progression through hydrolysis of cadherin extracellular domains with associated release of transcription factor(s). PMID: 21525715
  8. Effect of kallikrein 4 loss on enamel mineralization: comparison with mice lacking matrix metalloproteinase 20. PMID: 21454549
  9. Runx2 regulates the expression of ODAM and nuclear ODAM serves an important regulatory function in the mineralization of enamel through the regulation of MMP-20 apart from a different, currently unidentified, function of extracellular ODAM. PMID: 20665536
  10. expression of MMP-20 correlates with the stage-associated changes in the digestion of enamel proteins PMID: 12206593
  11. enamelysin activity is essential for proper enamel development in mice PMID: 12393861
  12. Enamelysin and collagen XVIII were co-localized in the developing enamel matrix and stratum intermedium and in the enamel-like tumor matrix of odontogenic tumors. PMID: 15296943
  13. Analyses of amelogenin mRNA between control and Mmp20(-/-)mice revealed no differences in the splicing pattern. PMID: 16998127
  14. The secretory-stage enamel organic extracellular matrix of Mmp20-/- mice showed a significant decrease in optical retardation as compared with Mmp20+/+ and Mmp20+/- mice (p=0.0000). PMID: 17364666
  15. Mice lacking expression of the AmelX, Enam and Mmp20 genes have been generated. PMID: 18714142

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Peptidase M10A family
Tissue Specificity
Expressed specifically in the enamel organ.
Database Links
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