Recombinant Mouse Mediator of DNA damage checkpoint protein 1 (Mdc1), partial

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

Mdc1

Uniprot No.

Q5PSV9

Alternative Names

Mdc1; Kiaa0170; Mediator of DNA damage checkpoint protein 1

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.

Gene References into Functions

MDC1 plays a fundamentally significant role in maintenance of genomic stability through a DNA damage response-independent pathway. PMID: 28193847
MDC1 silencing enhances the radiosensitivity of human nasopharyngeal cancer CNE1 cells and results in xenograft tumor growth inhibition. PMID: 26247734
oligomerization of MDC1 plays an important role in DDR and help understand the formation of proteins complexes at the sites of DNA damage. PMID: 21705321
structural insight into MDC1-CHK2 interaction PMID: 22211259
the DNA damage response pathway centered on MDC1 triggers epigenetic silencing of sex chromosomes in germ cells PMID: 21536735
Data show that loss of Mof leads to reduction of histone H4 K16 acetylation, cell cycle arrest, chromosome aberration, defects in DNA damage repair, and complete loss of Mdc1 response to DNA damage. PMID: 20837706
Data show that ATM-dependent resection at a subset of DSBs leads to ATR-dependent Chk1 activation, and that 53BP1(-/-) and MDC1(-/-) cells manifest a checkpoint defect at high radiation doses. PMID: 20421415
identification and description of a functional homologue of human MDC1/ NFBD1; in response to ionizing radiation it forms foci that co-localize with the MRE11-RAD50-NBS1 (MRN) complex and factors such as gammaH2AX and 53BP1 PMID: 15611643
MDC1, as a signal amplifier of the ATM pathway, is vital in controlling proper DNA damage response and maintaining genomic stability. PMID: 16427009
MDC1 knockdown affected the formation of telomere dysfunction-induced foci. PMID: 17158742
Dephosphorylation of TN-associated histone gammaH2AX chromatin suggests annealing of single strands or repair of double-strand breaks DSBs PMID: 17344431
Results show that MDC1 functions primarily in homologous recombination/sister chromatid recombination, in a manner strictly dependent upon its ability to interact with gamma-H2AX. PMID: 18158901
The MRE11-RAD50-Nijmegen breakage syndrome 1 (NBS1 [MRN]) complex accumulates at sites of DNA double-strand breaks (DSBs) in microscopically discernible nuclear foci in aregion in Mdc1. PMID: 18411308
MDC1 functions as an upstream regulator of 53BP1 in the DDR pathway and in tumor suppression PMID: 18504301
Data suggest that H2AX fuels a gamma-H2AX self-reinforcing mechanism that retains MDC1 and activated ATM in chromatin near DSBs and promotes continued local phosphorylation of H2AX. PMID: 19450528

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Subcellular Location

Nucleus. Chromosome.

Database Links

KEGG: mmu:240087

STRING: 10090.ENSMUSP00000080949

UniGene: Mm.218511

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Recombinant Mouse Mediator of DNA damage checkpoint protein 1 (Mdc1), partial

Product Details

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Target Background

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