Recombinant Mouse Mediator of RNA polymerase II transcription subunit 12 (Med12), partial

Code CSB-YP013643MO
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Source Yeast
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Code CSB-EP013643MO
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Source E.coli
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Code CSB-EP013643MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013643MO
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Source Baculovirus
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Code CSB-MP013643MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Med12
Uniprot No.
Alternative Names
Med12; Kiaa0192; Mopa; Tnrc11; Trap230; Mediator of RNA polymerase II transcription subunit 12; Mediator complex subunit 12; OPA-containing protein; Thyroid hormone receptor-associated protein complex 230 kDa component; Trap230; Trinucleotide repeat-containing gene 11 protein
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
Gene References into Functions
  1. Study provide evidence that Med12 regulates neural stem cell gene expression programs linked to adhesion and cell cycle progression but suppresses its adhesion by repressing the expression of cell adhesion molecules. PMID: 27188461
  2. alterations in MED12-dependent enhancer regulation may control both physiological and malignant hematopoiesis. PMID: 27570068
  3. The data reveal how the interplay between PRC1, ncRNA and Mediator complexes controls pluripotency and cellular differentiation. PMID: 27096886
  4. Med1 and Med12, two subunits of the mediator complex implicated in transcription initiation and long-range enhancer/promoter loop formation, are dynamically recruited to the IgH locus enhancers. PMID: 26903242
  5. leiomyomatous, Med12 c.131G>A variant-expressing uteri developed chromosomal rearrangements. PMID: 26193636
  6. this work points to Med12 being an essential coregulator of transcription factors controlling neural tube closure. PMID: 20589884
  7. show that in Med12 hypomorphic embryos, the Wnt/planar cell polarity pathway is disrupted and that canonical Wnt/beta-catenin signaling is impaired. PMID: 20630950
  8. Nanog and Med12 function in concert to regulate Nanog target genes and identify a novel role for Med12 in embryonic stem cell regulation. PMID: 19036726

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Subcellular Location
Nucleus.
Protein Families
Mediator complex subunit 12 family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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