Recombinant Mouse Microtubule-associated protein RP/EB family member 1 (Mapre1)

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

Mapre1

Uniprot No.

Q61166

Alternative Names

Mapre1Microtubule-associated protein RP/EB family member 1; APC-binding protein EB1; End-binding protein 1; EB1

Species

Mus musculus (Mouse)

Expression Region

2-268

Target Protein Sequence

AVNVYSTSV TSDNLSRHDM LAWINESLQL NLTKIEQLCS GAAYCQFMDM LFPGSIALKK VKFQAKLEHE YIQNFKILQA GFKRMGVDKI IPVDKLVKGK FQDNFEFVQW FKKFFDANYD GKEYDPVAAR QGQETAVAPS LVAPALSKPK KPLGSSTAAP QRPIATQRTT AAPKAGPGMV RKNPGVGNGD DEAAELMQQV KVLKLTVEDL EKERDFYFGK LRNIELICQE NEGENDPVLQ RIVDILYATD EGFVIPDEGG PQEEQEEY

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules. Acts as a regulator of autophagosome transport via interaction with CAMSAP2. May play a role in cell migration.

Gene References into Functions

Crosstalk between EB1 protein and Ankyrin-G is decisive for axon initial segment assembly. PMID: 27098687
We describe here a novel function for tau as a direct regulator of eb1 and eb2 proteins in differentiating neuronal cells PMID: 25761518
Data indicate that EB1(MAPRE1) is up-regulated in osteoblasts and is required for bone cell differentiation. PMID: 23740245
MAP1B interacts directly with EB1 and EB3 (EBs), two core 'microtubule plus-end tracking proteins' ( TIPs), and sequesters them in the cytosol of developing neuronal cells. PMID: 23572079
the Amer2-EB1-APC complex regulates cell migration by altering microtubule stability. PMID: 22898821
Detyrosination regulates CAP-Gly proteins interaction with alpha-tubulin, but not with EB1. PMID: 22432029
End binding protein 1 is required for single-cell polarization and directional cell motility. PMID: 22193958
These findings support a model where EB1 protein links microtubules to actin protrusion and cell polarity through signaling pathways involving PKC. PMID: 22120625
An EB1 mutant lacking the C-terminal tail could also fully rescue CLIP dissociation kinetics, but could only partially restore CLIP accumulation at the tips, suggesting that the interaction of CLIPs with the EB tails contributes to CLIP localization. PMID: 16148041
Localization of EB1 at the centriole/basal body is required for primary cilia assembly in fibroblasts. PMID: 17600711
EB1 was highly expressed in Sertoli cells and located along microtubule bundles in Sertoli cell processes,indicating a requirement of proper microtubule arrays for Sertoli cell plasticity and function. PMID: 17964570
Results suggest that EB1 favours the lateral association of free tubulin at microtubule-sheet edges, thereby stimulating nucleation, sheet growth and closure. PMID: 18364701
Results suggest that EB1 is necessary for the early stages of muscle differentiation. PMID: 19366726
EB1 coordinates melanoma cell migration through regulating the balance between lamellipodial and filopodial protrusion. PMID: 19427113

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Subcellular Location

Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Protein Families

MAPRE family

Tissue Specificity

Expressed within the midpiece of sperm tail (at protein level).

Database Links

KEGG: mmu:13589

STRING: 10090.ENSMUSP00000028981

UniGene: Mm.143877

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Recombinant Mouse Microtubule-associated protein RP/EB family member 1 (Mapre1)

Product Details

Customer Reviews and Q&A

Target Background

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