Recombinant Mouse Midnolin (Midn)

Code CSB-YP013822MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP013822MO
MSDS
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Source E.coli
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Code CSB-EP013822MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013822MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP013822MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Midn
Uniprot No.
Alternative Names
Midn; Midnolin; Midbrain nucleolar protein
Species
Mus musculus (Mouse)
Expression Region
1-465
Target Protein Sequence
MEPQPGGARS CRRGAPGGAC ELNTATESAA PMSLAIHSTT GTRYDLSVPH DETVEGLRKR LSQRLKVPKE RLALLHKDTR LSSGKLQEFG VGDGSKLTLV PTVEAGLMSQ ASRPEQSVMQ ALESLTETQV SDFLSGRSPL TLALRVGDHM MFVQLQLAAQ HAPLQHRHVL AAAAAAAAAA RGDSSVATPV SSPCRPVSSA ARVPPVSSSP SSPVSPSPVT AGSFRSHAAS TTCPEQMDCS PPASSSSTST PGSSPTPRSR KPGAVIESFV NHAPGVFSGT FSGTLHPNCQ DSSGRPRRDI GTILQILNDL LSATRHYQGM PPSLTQLRCH AQCSPASPAP DLTPKTTSCE KLAATSSTSL LQGQSQIRMC KPPGDRLRQT ENRATRCKVE RLQLLLQQKR LRRKARRDAR GPYHWTPSRK AGRSDSSSSG GGGGPSEATG LGLDFEDSVW KPEVNPDIQS EFVVA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Facilitates ubiquitin-independent proteasomal degradation of polycomb protein CBX4. Plays a role in inhibiting the activity of glucokinase GCK and both glucose-induced and basal insulin secretion.
Gene References into Functions
  1. midnolin plays a role in cellular signaling of adult tissues and regulates glucokinase enzyme activity in pancreatic beta cells PMID: 24187134
Subcellular Location
Nucleus. Cytoplasm, cytosol. Nucleus, nucleolus.
Tissue Specificity
Expressed at high levels in brain and liver with significantly lower levels in muscle.
Database Links

KEGG: mmu:59090

UniGene: Mm.143813

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