Recombinant Mouse Myotilin (Myot)

Code CSB-YP015364MO
MSDS
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Source Yeast
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Code CSB-EP015364MO
MSDS
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Source E.coli
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Code CSB-EP015364MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015364MO
MSDS
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Source Baculovirus
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Code CSB-MP015364MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Myot
Uniprot No.
Alternative Names
Myot; Myo; Ttid; Myotilin; Myofibrillar titin-like Ig domains protein; Titin immunoglobulin domain protein
Species
Mus musculus (Mouse)
Expression Region
1-496
Target Protein Sequence
MFNYERPKHF IQPQNPCGSR LQPPGPEVSG FPSQTKQSSI VIQPRQCTEQ RFSASSTVSS HITVSSSAYP APQQLAGPNP GQKVTATYNQ SPASFLSSIL PSQPDYCNSK IPSTVDSNYQ QSSVNQPVNA MSSQAANARP TPKTPDHEIQ GSKEALIQDL ERKLKCKDTL LHNGNQRLTY EEKMARRLLG PQNAAAVFQA QNSDVQDSPQ HNPEQARLHV PTSQVRSRSS SRAEANDQDA IQEKFYPPRF IQVPENMSIE EGRFCRMDFK VSGLPAPDVS WYLNGRPVQS DELHKMIVSE KGFHSLIFEV VRASDAGPYA CVARNRAGEA TFTVQLDVLA KEHKRAPMFI FKPQSKKVFE GETVKLECQI SAIPPPKLFW KRNNEMVQFN TDRISLYHDN AGRVTLLIKD VNKKDAGWYT VSAVNEAGVT TCNTRLDVTA RPIQTLPAPK QLRVRPTFSK YLALNGRGLD VKQAFNPEGE FQRLAAQSGL YESEEL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of a complex of multiple actin cross-linking proteins. Involved in the control of myofibril assembly and stability at the Z lines in muscle cells.
Gene References into Functions
  1. Myotilin does not have a significant influence on muscle mass, muscle fiber size, or regulation of muscle contraction. Alternatively, compensatory over-expressions of other elements may compensate for the lack of myotilin. PMID: 19878039
  2. features suggest an important role for myotilin in sarcomere organization. PMID: 15752755
  3. either myotilin does not participate in muscle development and basal function maintenance or other proteins serve as structural and functional compensatory molecules when myotilin is absent PMID: 17074808
  4. In single-transgenic mutant mice, double-transgenic mice overexpressing myotilin showed more severe muscle degeneration, enhanced myofibrillar aggregation, and earlier onset of aggregation. PMID: 18335471
Subcellular Location
Cell membrane, sarcolemma. Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line.
Protein Families
Myotilin/palladin family
Tissue Specificity
Expressed in skeletal muscle (at protein level).
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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