Recombinant Mouse Myotubularin-related protein 14 (Mtmr14)

Code CSB-YP823758MO
MSDS
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Source Yeast
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Code CSB-EP823758MO
MSDS
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Source E.coli
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Code CSB-EP823758MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP823758MO
MSDS
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Source Baculovirus
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Code CSB-MP823758MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mtmr14
Uniprot No.
Alternative Names
Mtmr14; Myotubularin-related protein 14; EC 3.1.3.-; mJumpy
Species
Mus musculus (Mouse)
Expression Region
1-648
Target Protein Sequence
MAGARAAAAA SAGSTASSGS PPPQEPGLWE LLEEFSRTQY RAKDSGGKSG SKVERIEKRC LELFGRDYCF SVIPNVNGDI CGHYPRHIVF LEYESSEKEK DTFQSTVQVN KLQDLIHRSK MARCRGRFVC PVILFKGKHI CRSATLAGWG ELYGRSGYNY LFSGGADDTW ASTEDVTEED FVLRSGDTHL FDKVRGYDIK LLQYLSVKYI CDLMVENKKV KFGMNVTSSE KVDKAQRYAN FTLLSIPYPG CEFFKEYKDR DYMAEGLIFN WKQDYVDAPL NIPNFLTQSL NIDWSQYQSW DLVQQTQNYL KLLLFIMNRD DDSGLLVHCI SGWDRTPLFI SLLRLSLWAD GLIHTSLKPA EILYLTVAYD WFLFGHMLVD RLSKGEEIFF FCFNFLKHIT SEEFCLKTQR RKSLPTRDAG FTVEDICMLR HKDRGSTTSL GSDFSLVLEH SPGAVGSFSY ETVELAPAGA PTQAAWRKSH SSSPQSMLWS RPQPSEERLP SHHGLTEAKS SSSSSSNHSD NFFRMGSSPL EVPKPRSVDH PLPGSSLSTD FGSWQLVSGC GSIQDRPVLH TDSSLPFSFQ DELPNSCLLT ALSDRETRLQ EVRSAFLAAY SSTVGLRAAT PSPSGAIGGL LEQFARGVGL RGTSTSTL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Lipid phosphatase which efficiently dephosphorylates phosphatidylinositol 3-phosphate (PtdIns3P) and PtdIns(3,5)P2; inactive toward PtdIns4P, PtdIns(3,4)P2, PtdIns(4,5)P2 and PtdIns(3,4,5)P3.
Gene References into Functions
  1. Data (including data from studies in knockout mice) suggest that Mtmr14 plays role in lipid metabolism and inflammation in liver, skeletal muscle, and adipose tissue in obesity induced by high-fat diet (HFD); here, Mtmr14 deficiency in knockout mice accelerates HFD-induced inflammation and metabolic dysfunction (obesity, hyperglycemia, and hyperlipidemia). PMID: 27807764
  2. propose that defect of MTMR14 promotes autophagy and cell proliferation in MEFs PMID: 24623267
  3. In young Mtmr14 KO mice (12 weeks). The T-tubule dysmorphology (swollen and elongated) could explain, in part, the defects in Ca2thorn excitationcontraction machinery observed in previous studies. PMID: 21930146
  4. a knockout mice were more susceptible to exercise-induced muscle damage, a trademark of muscle functional changes in older subjects PMID: 20817957
  5. MTMR14 may play an important role in muscle calcium homeostasis. PMID: 20834069
  6. Data report that mice deficient in the newly identified phosphatidylinositol phosphate phosphatase MIP/MTMR14 (muscle-specific inositol phosphatase) show muscle weakness and fatigue. PMID: 19465920

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Subcellular Location
Cytoplasm.
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class myotubularin subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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