Recombinant Mouse NGFI-A-binding protein 2 (Nab2)

Code CSB-YP730725MO
MSDS
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Source Yeast
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Code CSB-EP730725MO
MSDS
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Source E.coli
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Code CSB-EP730725MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP730725MO
MSDS
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Source Baculovirus
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Code CSB-MP730725MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nab2
Uniprot No.
Alternative Names
Nab2; NGFI-A-binding protein 2; EGR-1-binding protein 2
Species
Mus musculus (Mouse)
Expression Region
1-525
Target Protein Sequence
MHRAPSPTAE QPPGRGDNTR RTPQPRFKAS APAMALPRTL GELQLYRVLQ RANLLSYYET FIQQGGDDVQ QLCEAGEEEF LEIMALVGMA TKPLHVRRLQ KALREWATNP GLFSQPVPAV PVSSIPLFKI SETAGTRKGS MSNGHGSPGE KAGSARSFSP KSPLELGEKL SPLPGGPGAG DPRIWPGQST PESDVGAGGE EEAGSPPFSP PAGGGVSEGP GVGGVAAGGA GGGPDRLEPE MVRMVVESVE RIFRSFPRGD TGEIASLLKL NKKLARSVGH IFEMDDHDAQ KEEEIRKYSV IYGRLDSKRR EGKQLSLHEL TINEAAAQFC MRDNTLLLRR VELFSLSRQV ARESTYLSSL KGSRLHSEEL GGPPLKKLKQ EVGEQSHNEI QQPPPGPESY APPYRPSLEE DSASLSGESL DGHLQAVGSC PRLTPPPADL PLALPAHGLW SRHILQQTLM DEGLRLARLV SHDRVGRLSP CVPAKPPLAE FEEGLLDRCP APGPHPALVE GRRSSVKVEA EASRQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
Gene References into Functions
  1. These findings indicate a requirement for Nab2 in maintaining thymocyte number in male mice with age and in response to stress. PMID: 28282643
  2. Data suggest that histone deacetylase inhibitors inhibit the induction of NGF1A-binding protein Nab2 by brain derived neurotrophic factor (BDNF), and thereby the relative induction of dopamine and cyclic AMP-regulated phosphoprotein, 32 kDa (DARPP-32). PMID: 24204683
  3. Nab2 is a novel endogenous negative regulator of Egr-1-dependent TGF-beta signaling responsible for setting the intensity of fibrotic responses PMID: 19888474
  4. repression of the endogenous Rad gene by NAB2 involves interaction with the CHD4 (chromodomain helicase DNA-binding protein 4) subunit of the NuRD (nucleosome remodeling and deacetylase) chromatin remodeling complex PMID: 16574654
  5. We report here, that FGF23 induces not only Egr-1 but also two isoforms of NAB2, which are specific co-repressors of Egr-1. PMID: 17174939
  6. Helps control the fate of antigen recognition in T cells. PMID: 18203138

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Subcellular Location
Nucleus. Note=Isoform 2 is not localized to the nucleus.
Protein Families
NAB family
Tissue Specificity
Highly expressed in brain and thymus, and at lower levels in spleen, kidney, heart and testis. Isoform 1 is predominantly expressed in testis, whereas isoform 3 is more abundant in thymus.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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