Recombinant Mouse Neurofilament heavy polypeptide (Nefh), partial

Code CSB-YP015686MO
MSDS
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Source Yeast
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Code CSB-EP015686MO
MSDS
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Source E.coli
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Code CSB-EP015686MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015686MO
MSDS
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Source Baculovirus
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Code CSB-MP015686MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nefh
Uniprot No.
Alternative Names
Nefh; Kiaa0845; Nfh; Neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; Neurofilament triplet H protein
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Gene References into Functions
  1. Data indicate the presence of a HuD - BDNF - NF-H pathway activated as a regenerative response to the axonal damage induced by antiretroviral treatment to counteract the antiretroviral neurotoxicity. PMID: 27238228
  2. Microgravity causes substantial down-regulation of nerve tissue proteins, choline acetyltransferase, NF200, and calbindin in mouse motor neurons. PMID: 28091925
  3. Data conclude that NF-H and NF-M C-terminal domains do not normally regulate NF transport rates as previously proposed, but instead increase the proteolytic resistance of NF, thereby stabilizing the stationary neurofilament cytoskeleton along axons. PMID: 23028520
  4. plasma NfH levels closely reflect later stages of disease progression and therapeutic response in the SOD1(G93A) mouse model of amyotrophic lateral sclerosis PMID: 22815892
  5. NFH expression reveals an exquisite level of Cb stripe complexity that respects the transverse zone divisions and delineates an intricately patterned target field for Cb afferents. PMID: 20127431
  6. If expression of neurofilament heavy is developmentally delayed, the neurofilament network is established in a distal to proximal gradient, perhaps to allow distal axonal segments to develop prior to proximal segments. PMID: 20633607
  7. results obtained in mouse model for leprous neuropathy indicate biochemical alterations in neurofilaments, i.e., hyperphosphorylation of NF-H and NF-M PMID: 20306637
  8. Overexpression of peripherin in mice deficient for neurofilament light (NF-L) subunits induced a progressive ALS-like spinal motor neuron degeneration prevented by simultaneous overexpression of NF-H PMID: 12641746
  9. NF-H regulates the association of NFs with kinesin. Phosphorylation of NF-H dissociates NFs from kinesin and provides a mechanism by which NF-H phosphorylation can contribute to the slowing of NF axonal transport. PMID: 16246456
  10. Alpha-internexin coassembles with all 3 neurofilament proteins into a single network of filaments in quadruple-transfected cells. Deleting NF-M alone or together with NF-H in mice reduces alpha-internexin transport & content in axons throughout the CNS. PMID: 17005864
  11. We found an amacrine cell type that was immunolabeled with an antibody against SMI32, a non-phosphorylated epitope on neurofilament proteins of high molecular weight, in the mouse retina. PMID: 17723270
  12. We have validated the serum pNF-H assay as an unbiased measurement of axonal injury in EAE, facilitating rapid screening of potential neuroprotective therapies in this model. PMID: 18709652

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Subcellular Location
Cytoplasm, cytoskeleton. Cell projection, axon.
Protein Families
Intermediate filament family
Tissue Specificity
Expressed in the sciatic nerve (at protein level).
Database Links
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