Recombinant Mouse POU domain, class 4, transcription factor 3 (Pou4f3)

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

Pou4f3

Uniprot No.

Alternative Names

Pou4f3; Brn-3.1; Brn-3c; Brn3c; POU domain; class 4; transcription factor 3; Brain-specific homeobox/POU domain protein 3C; Brain-3C; Brn-3C; Brn-3.1

Species

Mus musculus (Mouse)

Expression Region

1-338

Target Protein Sequence

MMAMNAKQPF GMHPVLQEPK FSSLHSGSEA MRRVCLPAPQ LQGNIFGSFD ESLLARAEAL AAVDIVSHGK NHPFKPDATY HTMSSVPCTS TSPTVPISHP AALTSHPHHA VHQGLEGDLL EHISPTLSVS GLGAPEHSVM PAQIHPHHLG AMGHLHQAMG MSHPHAVAPH SAMPACLSDV ESDPRELEAF AERFKQRRIK LGVTQADVGA ALANLKIPGV GSLSQSTICR FESLTLSHNN MIALKPVLQA WLEEAEAAYR EKNSKPELFN GSERKRKRTS IAAPEKRSLE AYFAIQPRPS SEKIAAIAEK LDLKKNVVRV WFCNQRQKQK RMKYSAVH

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Acts as a transcriptional activator. Acts by binding to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes. Involved in the auditory system development, required for terminal differentiation of hair cells in the inner ear.

Gene References into Functions

identify separate enhancers at various locations of pou4f3 gene that direct expression to different inner ear hair cells types at different ages and determine that 0.4 kb of upstream sequence determines expression level PMID: 27592349
Atoh1 function in hair cell differentiation is modulated by interaction with other transcription factors, such as Gfi1 and Pou4f3. (Review) PMID: 27751776
These data illuminate a genetic pathway that initiates auditory HC regeneration and suggest p27(Kip1), GATA3, and POU4F3 as additional therapeutic targets for ATOH1-mediated auditory hair cells regeneration. PMID: 28402854
These data demonstrate that Nr2f2 is a direct target of POU4F3 in vitro and that this regulatory relationship may be relevant to hair cell development and survival. PMID: 25372459
induction of POU4F3 by TFE2 and GATA3 PMID: 22985730
Data show that Ap-2delta occupies and activates the Pou4f3 and Bhlhb4 promoters. PMID: 21858141
Stress-granule-associated protein Caprin-1 is downregulated by Pou4f3. PMID: 21402877
Taken together, our data suggest that SHH plays an important role in the promotion of auditory hair cell differentiation via the Math1-Brn3.1 signaling pathway. PMID: 19908278
Brn3c null mice show severe compromised production of these neurotrophins and should therefore show a comparable pattern of nerve fiber loss. PMID: 12585968
Brn-3c was found to contain an independent N-terminal activation domain that is sufficient to activate gene transcription in the organ-of-Corti-derived cell lines. PMID: 16226339
Inner hair cells constitutively express alpha9alpha10 nicotinic cholinergic receptors into adulthood by expressing the alpha10 cDNA under the control of the Pou4f3 gene promoter. PMID: 19452222

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

POU transcription factor family, Class-4 subfamily

Tissue Specificity

Brain.

Database Links

KEGG: mmu:18998

STRING: 10090.ENSMUSP00000025374

UniGene: Mm.488204

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Recombinant Mouse POU domain, class 4, transcription factor 3 (Pou4f3)

Product Details

Customer Reviews and Q&A

Target Background

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