Recombinant Mouse Peroxisomal targeting signal 1 receptor (Pex5)

Code CSB-YP017805MO
MSDS
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Source Yeast
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Code CSB-EP017805MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017805MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP017805MO
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pex5
Uniprot No.
Alternative Names
Pex5; Pxr1; Peroxisomal targeting signal 1 receptor; PTS1 receptor; PTS1R; PTS1-BP; PXR1P; Peroxin-5; Peroxisomal C-terminal targeting signal import receptor; Peroxisome receptor 1
Species
Mus musculus (Mouse)
Expression Region
1-639
Target Protein Sequence
MAMRELVEGE CGGANPLMKL ATHFTQDKAL RQEGLRPGPW PPGASAAETV SKPLGVGTED ELVSEFLQDQ NATLVSRAPQ TFKMDDLLAE MQEIEQSNFR QAPQRAPGVA DLALSENWAQ EFLAAGDAVD VAQDYNETDW SQEFIAEVTD PLSVSPARWA EEYLEQSEEK LWLGDQEGSS TADRWYDEYH PEEDLQHTAS DFVSKVDDPK LANSEFLKFV RQIGEGQVSL ESAAGSGGAQ AEQWAAEFIQ QQGTSEAWVD QFTRPGNKIA ALQVEFERAK SAIESDVDFW DKLQAELEEM AKRDAEAHPW LSDYDDLTSA SYDKGYQFEE ENPLRDHPQP FEEGLHRLEE GDLPNAVLLF EAAVQQDPKH MEAWQYLGTT QAENEQELLA ISALRRCLEL KPDNRTALMA LAVSFTNESL QRQACETLRD WLRYSPAYAH LVAPGEEGAT GAGPSKRILG SLLSDSLFLE VKDLFLAAVR LDPTSIDPDV QCGLGVLFNL SGEYDKAVDC FTAALSVRPN DYLMWNKLGA TLANGNQSEE AVAAYRRALE LQPGYIRSRY NLGISCINLG AHREAVEHFL EALNMQRKSR GPRGEGGAMS ENIWSTLRLA LSMLGQSDAY GAADARDLSA LLAMFGLPQ
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import.
Gene References into Functions
  1. monoubiquitination of the N-terminal cysteine of peroxisome-associated PEX5 not only functions to recycle the peroxin back to the cytosol, but also serves as a quality control mechanism to eliminate peroxisomes with a defective protein import machinery PMID: 26086376
  2. Demyelination in cerebellum and brain stem preceded major myelin loss in corpus callosum of Pex5 deficient mice. PMID: 22458306
  3. interaction of PEX5 with catalase and PEX14 PMID: 21976670
  4. Absence of peroxisomal metabolism in neurons and astrocytes does not provoke the neurodegenerative phenotype observed after deleting peroxisomes from oligodendrocytes. Lack of peroxisomal metabolism in astrocytes causes increased VLCFA levels in myelin PMID: 20578053
  5. Peroxisomes were selectively reconstituted in brain or liver of Pex5 knock-out mice, a model for Zellweger syndrome, by tissue-selective overexpression of Pex5p. We found that both rescue strains exhibited a correction of the neuronal migration defect PMID: 14586000

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Subcellular Location
Cytoplasm. Peroxisome membrane; Peripheral membrane protein. Note=Its distribution appears to be dynamic. It is probably a cycling receptor found mainly in the cytoplasm and as well associated to the peroxisomal membrane through a docking factor (PEX13).
Protein Families
Peroxisomal targeting signal receptor family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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