Recombinant Mouse Phosphatidylinositol 4-kinase type 2-beta (Pi4k2b)

Code CSB-YP804016MO
MSDS
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Source Yeast
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Code CSB-EP804016MO
MSDS
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Source E.coli
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Code CSB-EP804016MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP804016MO
MSDS
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Source Baculovirus
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Code CSB-MP804016MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pi4k2b
Uniprot No.
Alternative Names
Pi4k2b; Phosphatidylinositol 4-kinase type 2-beta; EC 2.7.1.67; Phosphatidylinositol 4-kinase type II-beta
Species
Mus musculus (Mouse)
Expression Region
1-469
Target Protein Sequence
MAEACEPTRP SEDEDEEREP LLPRVAWAQP RRVAPGSAVR MQADEGADVL REPATDEPPA VSGEGSISAS LSTELDRTRT TSSETNTFLE DPEFADIVLK AEQAIEIGVF PERISQGSSG SYFVKDSKRN IIGVFKPKSE EPYGQLNPKW TKYVHKVCCP CCFGRGCLLP NQGYLSEAGA YLVDVKLNLG IVPKTKVVWL VSETFNYSAI DRAKSRGKKY ALEKVPKVGR KFHRIGLPPK VGSFQLFVKD YKEAEYWLRR FEAEPLPENI RKQFQSQFEK LVILDYIIRN TDRGNDNWLV KYDEMKYAKK IESEESNWID NKQLLIKIAA IDNGLAFPFK HPDEWRAYPF HWAWLPQAKV PFSEETRNLI LPYISDMNFV QDLCEDLYEL FKTDKGFDRA AFENQMSVMR GQILNLTQAL RDGKSPMQLA QMPCVIVECS KSGSQGRVVH LGSSFTQTVH CRKPFFSSW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking.
Gene References into Functions
  1. UV irradiation increases nuclear PtdIns5P levels via inhibition of the activity of the beta isoform of PtdIns5P 4-kinase (PIP4Kbeta), an enzyme that can phosphorylate and remove PtdIns5P. PMID: 16949365
Subcellular Location
Cytoplasm. Membrane.
Protein Families
PI3/PI4-kinase family, Type II PI4K subfamily
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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