Recombinant Mouse Probable E3 ubiquitin-protein ligase makorin-3 (Mkrn3)

Code CSB-YP736743MO
MSDS
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Source Yeast
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Code CSB-EP736743MO
MSDS
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Source E.coli
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Code CSB-EP736743MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP736743MO
MSDS
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Source Baculovirus
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Code CSB-MP736743MO
MSDS
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Source Mammalian cell
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Product Details

Purity
≥85% (SDS-PAGE)
Target Names
Mkrn3
Uniprot No.
Alternative Names
Mkrn3; Zfp127; Znf127; Probable E3 ubiquitin-protein ligase makorin-3; EC 2.3.2.27; RING-type E3 ubiquitin transferase makorin-3; Zinc finger protein 127
Species
Mus musculus (Mouse)
Expression Region
1-544
Target Protein Sequence
MEESTAPIEA HAAAGAEAGA EGGEGVSVPP PPQFEAAGAS AGVSSAPLQQ ASGLAPLLVT PGPAIRRAAS LRPAPAEGGG ARSGPERNSG SWTKQILCRY YLHGQCKEGD NCRYSHDLSG RRRSRGGQDA QPRASADRGP KMATRWEPPT QEVAEAPPAA SSSSLPLIGS AAERGFTEAE IDNAGIRSAA ERGFSEAEID NASLAAGAAA GAGAEGWEGA IEFVPGQPYR GRMVPPHGPE APLQSPAIER EHMAMGMGMP MPVPMPMPVP MPVPMPLPLC RYAARGQCLR GDRCAYPHGE ICDMCGQQAL HPWDAAQQEA HRRACVEAHE RDMELSFAVQ RSMDKVCGIC MEVVYEKADP SDRRFGILFS CNHTYCLRCI RRWRSATQFE NRISKSCPQC RVSSGFVIPS EFWVEEEEEK EKLVQQYKEG MSQKACRYFA GGLGHCPFGE FCFYKHEYPE GWRDQPPRPD GGGSSSAYWH QVLEPVQLRE GNVLFKSRKK EHSVLRLANQ LLKKLLCLRG SSSFSDDRWL LLQYQLEEYF SLNL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.
Gene References into Functions
  1. Zfp127 is a novel regulator of Oct4, and may become a potent target to improve cellular reprogramming. PMID: 29335068
  2. Mkrn3, encoding a RING zinc-finger protein, is imprinted, with preferential expression from the paternal allele. PMID: 10196368
Tissue Specificity
Expressed in testis, brain, heart and kidney. Ubiquitously detected at low levels throughout the entire embryo, but expression is highest in the ventricular layers of the brain.
Database Links

KEGG: mmu:22652

STRING: 10090.ENSMUSP00000091898

UniGene: Mm.146

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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