Recombinant Mouse Proteasome activator complex subunit 3 (Psme3)

Code CSB-YP018918MO
MSDS
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Source Yeast
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Code CSB-EP018918MO
MSDS
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Source E.coli
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Code CSB-EP018918MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018918MO
MSDS
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Source Baculovirus
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Code CSB-MP018918MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Psme3; Proteasome activator complex subunit 3; 11S regulator complex subunit gamma; REG-gamma; Activator of multicatalytic protease subunit 3; Ki nuclear autoantigen; Proteasome activator 28 subunit gamma; PA28g; PA28gamma
Species
Mus musculus (Mouse)
Expression Region
2-254
Target Protein Sequence
ASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA ETLY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition.
Gene References into Functions
  1. the ubiquitin-independent REGgamma proteasome regulates energy homeostasis PMID: 27511885
  2. Study demonstrated that Psme3/Psme4 double KO (dKO) male mice are completely infertile and showed the up-regulation of several proteins involved in oxidative stress response in dKO sperms. These results suggest the important role of PA28gamma and PA200 in male fertility. PMID: 27003159
  3. Findings reveal a novel regulatory pathway in which the REGgamma-proteasome controls the steady-state level of GSK3beta protein. PMID: 26370326
  4. PSME3 is a target gene of NF-kappaB during bacterial infections. PSME3 activates NF-kappaB by degrading KLF2. PMID: 26776519
  5. REGgamma-mediated control of IkappaBvarepsilon contributes to NFkappaB activation and promotes bowel inflammation and associated tumour formation in response to chronic injury PMID: 26899380
  6. REGgamma acts in skin tumorigenesis mediating MAPK/p38 activation of the Wnt/beta-catenin pathway. PMID: 25908095
  7. In a p53-dominated cellular context, pro-apoptotic signaling might be overcome by PA28gamma-mediated caspase inhibition. PMID: 26201457
  8. Data suggest levels of gene expression of both Psme3 (proteasome activator subunit 3) and Dusp3 (dual specificity phosphatase 3) are associated with susceptibility to Staphylococcus aureus infection/sepsis in humans and in mouse disease model. PMID: 24901344
  9. REGgamma binds to SirT1, promotes its Ub-independent degradation, and inhibits its activity to deacetylate autophagy-related proteins, thereby inhibiting autophagy under normal conditions. PMID: 24011073
  10. REGgamma-deficient mice develop premature aging phenotypes that are associated with abnormal accumulation of casein kinase (CK)1delta and p53. Antibody array analysis identified CK1delta as a direct target of REGgamma. PMID: 23766372
  11. the regulation of REGgamma assembly and activity, suggesting a potential venue for the intervention of the ubiquitin-independent REGgamma proteasome activity. PMID: 23612972
  12. PA28gamma acts as a co-repressor of HTLV-1 p30 to suppress virus replication and is required for the maintenance of viral latency. PMID: 23104922
  13. PA28gamma stimulates MAFA degradation through a novel molecular mechanism that is distinct from that for the degradation of p21 PMID: 21830322
  14. Plasma cell differentiation to enable antibody secretion resulted in a transiently upregulated proteasome activator (PA) 28gamma, a component of the putative nuclear proteasome. PMID: 21319304
  15. REGgamma regulates cellular distribution of p53 by facilitating its multiple monoubiquitylation and nuclear export. PMID: 21084564
  16. REGgamma is present in many tissues and the highest expression is in the testis. PMID: 20494959
  17. Loss of PA28g expression in 28-kDA proteasome activator gamma-deficient mice results in antigen-specific defects in the processing and presentation of MHC class I-restricted T cell epitopes. PMID: 15004203
  18. REGgamma is not a viable therapeutic target in polyglutamine disease and that overall proteasome function is not impaired by trapped mutant polyglutamine in (Huntington's disease) R6/2 mice. PMID: 16311253
  19. PA28gamma plays a crucial role in the development of liver pathology induced by HCV infection PMID: 17234812

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Subcellular Location
Nucleus. Cytoplasm. Note=Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase.
Protein Families
PA28 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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