This recombinant mouse protein PML is produced in an E. coli expression system and covers amino acids 60-243. The protein includes an N-terminal 10xHis-tag and a C-terminal Myc-tag, which should make purification and detection more straightforward. It comes with purity greater than 85% based on SDS-PAGE analysis, though this level appears adequate for most research applications. Important to note: this product is strictly for research use and cannot be used for diagnostic or therapeutic purposes.
PML protein plays what seems to be a critical role in cellular processes like apoptosis and senescence. It's involved in forming nuclear bodies, structures that appear essential for various pathways including DNA repair and antiviral defense mechanisms. Given its involvement in these processes, PML has become a significant focus in research areas related to cell cycle regulation and cancer biology.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Protein-Protein Interaction Studies
This dual-tagged PML fragment (covering amino acids 60-243) works well in pull-down assays to identify and characterize protein interactions within the central region of mouse PML. The N-terminal His-tag allows for immobilization on nickel-based resins. Meanwhile, the C-terminal Myc-tag provides a way to detect and validate binding partners through Western blot analysis. This particular region may contain important interaction domains that researchers can study separately from the complexity of full-length PML.
2. Antibody Development and Validation
The recombinant PML fragment serves as a practical immunogen or screening antigen when developing antibodies specific to the 60-243aa region of mouse PML. Its dual tagging system makes purification and detection relatively simple during antibody production workflows. Researchers might find this protein useful for generating region-specific antibodies to study PML localization, expression patterns, or modifications in mouse cell lines and tissues.
3. Structural and Biochemical Characterization
This defined PML fragment can be used for biophysical studies aimed at understanding the structural properties of the 60-243aa region. Such studies might include secondary structure analysis, thermal stability testing, and folding characteristics. The high purity (>85%) and dual tags should make purification and handling easier for techniques like circular dichroism spectroscopy, dynamic light scattering, or analytical ultracentrifugation. These approaches could provide insights into how this specific PML domain is structurally organized.
4. ELISA-Based Quantitative Assays
Both the Myc and His tags open up possibilities for developing sandwich or competitive ELISA formats in quantitative detection applications. The protein can function as a standard or competitor in assays designed to measure PML levels or investigate PML-related pathways in mouse cell extracts or experimental samples. Having dual tags gives researchers some flexibility in how they design assays and choose detection strategies.
