Recombinant Mouse Protein shisa-9 (Shisa9), partial

Code CSB-YP866643MO
MSDS
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Source Yeast
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Code CSB-EP866643MO
MSDS
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Source E.coli
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Code CSB-EP866643MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866643MO
MSDS
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Source Baculovirus
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Code CSB-MP866643MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Shisa9
Uniprot No.
Alternative Names
Shisa9; Protein shisa-9; Cystine-knot AMPAR modulating protein of 44 kDa; CKAMP44
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulator of short-term neuronal synaptic plasticity in the dentate gyrus. Associates with AMPA receptors (ionotropic glutamate receptors) in synaptic spines and promotes AMPA receptor desensitization at excitatory synapses.
Gene References into Functions
  1. Data indicate the importance of CKAMP44 for modulating synaptic short-term depression and visual input integration. PMID: 29343769
  2. Ckamp44 expression is strongest in dentate gyrus granule cells and less abundant in the rest of the brain PMID: 26645823
  3. coexpression of both auxiliary subunits,CKAMP44 and TARP gamma-8 is necessary for the efficient targeting of AMPARs to the cell surface of dentate gyrus granule cells PMID: 25066086
  4. Data show interaction partners of AMPA receptor auxiliary subunit Shisa9, and that the C-terminal interactions of Shisa9 through its PDZ domain interaction motif are important for AMPA receptor synaptic and network functions. PMID: 24498314
  5. CKAMP44 identified as a brain-specific type I transmembrane protein that associates with AMPA receptors in synaptic spines; findings show that CKAMP44 modulates short-term plasticity at specific excitatory synapses PMID: 20185686

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Subcellular Location
Cell projection, dendritic spine membrane; Single-pass type I membrane protein. Cell junction, synapse.
Protein Families
Shisa family, SHISA9 subfamily
Tissue Specificity
Brain-specific. Mainly expressed in neurons, including in hippocampus, cerebral cortex, striatum, thalamus, olfactory bulb and cerebellum. Expressed in most brain structures during embryonic and postnatal development.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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