Recombinant Mouse Ras-related GTP-binding protein D (Rragd)

Code CSB-YP749277MO
MSDS
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Source Yeast
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Code CSB-EP749277MO
MSDS
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Source E.coli
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Code CSB-EP749277MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP749277MO
MSDS
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Source Baculovirus
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Code CSB-MP749277MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rragd
Uniprot No.
Alternative Names
Rragd; Ragd; Ras-related GTP-binding protein D; Rag D; RagD
Species
Mus musculus (Mouse)
Expression Region
1-449
Target Protein Sequence
MSQVLGKPQP QGEDGGEDQE EDELVGLAGY EDGPESSDAE LDSGPEEGES RRNSWMPRSW CSEATRHECW EPGLWRSSHL LGIGGGWRML RRQRQADFFL DFSDPFSTEV KPRILLMGLR RSGKSSIQKV VFHKMSPSET LFLESTNRIC REDVSNSSFV NFQIWDFPGQ IDFFDPTFDY EMIFRGTGAL IFVIDSQDDY MEALARLHLT VTRAYKVNTD INFEVFIHKV DGLSDDHKIE TQRDIHQRAN DDLADAGLEK IHLSFYLTSI YDHSIFEAFS KVVQKLIPQL PTLENLLNIF ISNSGIEKAF LFDVVSKIYI ATDSTPVDMQ TYELCCDMID VVIDISCIYG LKEDGAGAPY DKDSTAIIKL NNTTVLYLKE VTKFLALVCF VREESFERKG LIDYNFHCFR KAIHEVFEVR MKMVKSRKAQ SRLPKKTGAT PNGTPRVLL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGA or RRAGB and cycles between an inactive GTP-bound and an active GDP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
Subcellular Location
Cytoplasm. Nucleus. Lysosome.
Protein Families
GTR/RAG GTP-binding protein family
Database Links

KEGG: mmu:52187

UniGene: Mm.300814

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