Recombinant Mouse Ras-related protein Rab-34 (Rab34)

Code CSB-YP734076MO
MSDS
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Source Yeast
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Code CSB-EP734076MO
MSDS
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Source E.coli
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Code CSB-EP734076MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP734076MO
MSDS
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Source Baculovirus
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Code CSB-MP734076MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rab34
Uniprot No.
Alternative Names
Rab34; Rab39; Rah; Rah1; Ras-related protein Rab-34; Ras-related homolog; Ras-related protein Rab-39; Ras-related protein Rah
Species
Mus musculus (Mouse)
Expression Region
1-259
Target Protein Sequence
MNILAPVRRD RVLAELPQCL KKEAALHVRK DFHPRVTCAC QEHRTGTVGF KISKVIVVGD LSVGKTCLIN RFCKDTFDKN YKATIGVDFE MERFEVLGVP FSLQLWDTAG QERFKCIAST YYRGAQAIII VFNLNDVASL EHTKQWLTDA LKENDPSNVL LFLVGSKKDL STPAQYSLME KDALKVAQEI KAEYWAVSSL TGENVREFFF RVAALTFEAN VLADVEKSGA RHIADVVRIN SDDKNLYLTA SKKKATCCP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transport protein involved in the redistribution of lysosomes to the peri-Golgi region. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Acts also as a positive regulator of hedgehog signaling and regulates ciliary function.
Gene References into Functions
  1. CSN6 and Rab34 are involved in AR trafficking by regulating the phosphorylation signaling pathway. These findings provide new insights into the testosterone signaling pathway in Sertoli cells that mediates spermatogenesis. PMID: 29991022
  2. Loss-of-function mutations in the Rab34 gene lead to defects in the assembly of primary cilia, in the trafficking of proteins to primary cilia and, consequently, to impaired reception of signals from the Hedgehog pathway. PMID: 29290584
  3. Although Rab34 regulates both lysosomal positioning and lysosome to phagosome fusion, the latter effect is not due to the regulation of the cytoplasmic accessibility of lysosomes to phagosomes by Rab34. PMID: 23871933
  4. uncover Rab34/Munc13-2 as a critical part of an alternative Rab7-independent phagosome maturation machinery and lysosome-mediated killing of mycobacteria PMID: 23197834
  5. association with membrane ruffles and macropinosomes and promotes macropinosome formation PMID: 12446704
  6. results have uncovered a novel mechanism for interorganellar trans-regulation of spatial distribution of lysosomes by Golgi-associated Rab34 PMID: 12475955

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Subcellular Location
Cytoplasm. Golgi apparatus. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane; Lipid-anchor; Cytoplasmic side. Cell projection, cilium.
Protein Families
Small GTPase superfamily, Rab family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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