Recombinant Mouse Receptor-binding cancer antigen expressed on SiSo cells (Ebag9), partial

Code CSB-YP871949MO
MSDS
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Source Yeast
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Code CSB-EP871949MO
MSDS
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Source E.coli
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Code CSB-EP871949MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP871949MO
MSDS
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Source Baculovirus
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Code CSB-MP871949MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ebag9
Uniprot No.
Alternative Names
Ebag9; Rcas1Receptor-binding cancer antigen expressed on SiSo cells; Cancer-associated surface antigen RCAS1; Estrogen receptor-binding fragment-associated gene 9 protein
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Gene References into Functions
  1. EBAG9 overexpression can be causative in enhancing the malignant properties of tumor cells. PMID: 21573489
  2. In the RCAS1 siRNA group, blood pressure was raised whereas urine albumin/creatinine ratio was increased. Our results suggest the importance of RCAS1 protein in the pathophysiologic condition of preeclampsia PMID: 20579953
  3. findings show RCAS1 induced cytochrome c release & activation of caspase-3 for apoptosis; cyclin D3 decreased significantly; results suggest cyclin D3 is one of key target molecules in RCAS1-RCAS1-R signaling pathway PMID: 20372814
  4. In normal pregnant mice, RCAS1 protein mRNA was significantly increased on day 7.5 p.c. Antigen localization was detected in the placenta, decidua, and fetus. PMID: 20015327
  5. EBAG9 may have a role in promoting progression of bladder cancer PMID: 19030177
  6. EBAG9 promoted primary 4T1 mammary carcinoma growth and distant metastasis, and EBAG9 small interfering RNA exerted overt regression of tumor growth and metastasis PMID: 19445026
  7. EBAG9 is a tunable inhibitor of CTL-mediated adaptive immune response functions. PMID: 19620783

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Subcellular Location
Golgi apparatus membrane; Single-pass type III membrane protein.
Tissue Specificity
Widely expressed. Expressed in heart, brain, spleen, liver, kidney and testis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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