Recombinant Mouse Rho guanine nucleotide exchange factor 4 (Arhgef4)

Code CSB-YP766503MO
MSDS
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Source Yeast
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Code CSB-EP766503MO
MSDS
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Source E.coli
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Code CSB-EP766503MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP766503MO
MSDS
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Source Baculovirus
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Code CSB-MP766503MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Arhgef4
Uniprot No.
Alternative Names
Arhgef4; Kiaa1112Rho guanine nucleotide exchange factor 4; APC-stimulated guanine nucleotide exchange factor; Asef
Species
Mus musculus (Mouse)
Expression Region
1-484
Target Protein Sequence
MDDQELGFKA GDVIEVMDAT NREWWWGRVA DGEGWFPASF VRLRVNQDEP ADDYEAPRAG AREADDSGPE AQSCKDQMRT NVINEILSTE RDYIKHLRDI CEGYVRQCRK REDMFSEEQL RTIFGNIEDI YRCQKAFVKA LEQKFNTERP HLSELGACFL EHQADFQIYS EYCNNHPNAC VELSRLTKLS KYVYFFEACR LLQRMIDISL DGFLLTPVQK ICKYPLQLAE LLKYTHPQHR DFKNVEAALH AMKNVAQLIN ERKRRLENID KIAQWQSSIE DWEGEDLLVR SSELIHSGEL TRVTQPQARS QQRMFFLFDR QLIYCKKDLL RRDVLYYKGR LDMDDLEVVD VEDGKDRDLH VSVKNAFRLY CGTTGDSHLL CARKPEQKQR WLKAFARERE QVRLDQETGF SITELQRKQA MLNASKQQAT GKPKAVGRPG YLTRHKHPSL PASRPQQQVL VLAEPRRKPS NFWHSISRLA PFRK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
Gene References into Functions
  1. Asef has a role in HGF-mediated protection against endothelial hyperpermeability and lung injury. PMID: 25518936
  2. these results demonstrate potent anti-inflammatory effects by HGF and delineate a key role of Asef in the mediation of the HGF barrier protective and anti-inflammatory effects. PMID: 25539852
  3. Asef and Asef2 are required for adenoma formation in Apc(Min/+)mice. PMID: 19893577
Subcellular Location
Cytoplasm. Note=Colocalized with APC in the synapse of olfactory glomerulus.
Tissue Specificity
Expressed in colon epithelial cells. Highly expressed in CNS, including hippocampus, olfactory bulb and cerebellum. Expression is aberrantly enhanced in most colorectal tumors.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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