Product Details

Purity

>85% (SDS-PAGE)

Target Names

Rnd3

Uniprot No.

P61588

Alternative Names

Rnd3; Arhe; Rhoe; Rho-related GTP-binding protein RhoE; Rho family GTPase 3; Rnd3

Species

Mus musculus (Mouse)

Expression Region

1-241

Target Protein Sequence

MKERRASQKL SSKSIMDPNQ NVKCKIVVVG DSQCGKTALL HVFAKDCFPE NYVPTVFENY TASFEIDTQR IELSLWDTSG SPYYDNVRPL SYPDSDAVLI CFDISRPETL DSVLKKWKGE IQEFCPNTKM LLVGCKSDLR TDVSTLVELS NHRQTPVSYD QGANMAKQIG AATYIECSAL QSENSVRDIF HVATLACVNK TNKNVKRNKS QRATKRISHM PSRPELSAVA TDLRKDKAKS C

Protein Length

Full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins.

Gene References into Functions

Rnd3 acted as a novel proangiogenic factor involved in cardiac responsive angiogenesis through HIF1alpha-VEGFA signaling promotion. PMID: 26781283
These results indicate that RhoE plays a role in suppressing tumor initiation and progression. PMID: 26036260
Rnd3 is a novel factor involved in intracellular calcium homeostasis regulation in the heart. PMID: 25348166
Animals with Rnd3 haploinsufficiency are predisposed to hemodynamic stress. Hyperactivation of Rho kinase activity is responsible in part for the apoptotic cardiomyopathy development. PMID: 24901055
Data show that the Plexin B2 receptor interacts physically and functionally with Rnd3 and stimulates RhoA activity in migrating cortical neurons. PMID: 24572910
RhoE is indispensable for osteoclast migration and bone resorption by maintaining fast actin turnover in podosomes. RhoE activates podosome component cofilin by inhibiting its Rock-mediated phosphorylation. PMID: 24284899
although Rnd3 expression is controlled transcriptionally by Ascl1, this proneural factor is itself required in radial glial progenitors only for proper orientation of cell divisions PMID: 23535656
Rnd3 is a regulator of Notch1 signaling PMID: 23630292
These findings support our previous report revealing the important role of RhoE in the normal development of the nervous system PMID: 22428561
the important role of Rnd3/RhoE in the normal development PMID: 21552537
a well defined Rnd3 effector Syx PMID: 20811643
RhoE induces stress fiber disassembly by directly binding ROCK I and inhibiting it from phosphorylating downstream targets PMID: 12773565
RhoE inhibits cell cycle progression upstream of the pRb checkpoint. PMID: 15340047
study shows RhoE physiological upregulation before myoblast fusion is responsible for the decrease in RhoA and ROCKI activities, which are required for the fusion process; therefore, we conclude that RhoE is an essential regulator of myoblast fusion PMID: 18369372
Our results suggest that RhoE can play important roles in the postnatal development and maturation of the CNS, especially in the migratory processes affecting the neurones. PMID: 19589369
RhoE inhibits 4E-BP1 phosphorylation and eIF4E function impairing cap-dependent translation. PMID: 19850923

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Subcellular Location

Golgi apparatus membrane; Peripheral membrane protein.

Protein Families

Small GTPase superfamily, Rho family

Tissue Specificity

Ubiquitous.

Database Links

KEGG: mmu:74194

STRING: 10090.ENSMUSP00000017288

UniGene: Mm.46497

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Recombinant Mouse Rho-related GTP-binding protein RhoE (Rnd3)

Product Details

Customer Reviews and Q&A

Target Background

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