Recombinant Mouse Roundabout homolog 1(Robo1),partial

Code CSB-YP020054MO
Size US$1916
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Robo1
Uniprot No. O89026
Research Area Cancer
Alternative Names Robo1; Dutt1; Roundabout homolog 1
Species Mus musculus (Mouse)
Source Yeast
Expression Region 880-1612aa
Target Protein Sequence YRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPAPMYLQQDELQEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTRQPKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 82.0 kDa
Protein Length Partial
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development
Gene References into Functions
  1. Low Robo1 expression is associated with developmental defects of cranial frontal and parietal bones. PMID: 28987541
  2. Contralateral migration of oculomotor neurons is regulated by Slit/Robo signaling. Results demonstrate that a migratory subset of motor neurons respond to floor plate-derived Slit repulsion to properly control the timing of contralateral migration. PMID: 27770832
  3. While Slit1 and Robo2 are only expressed in peripheral axons and their cell bodies, Slit2, Slit3 and Robo1 are also expressed in satellite cells of the dorsal root ganglion, Schwann cells and fibroblasts of peripheral nerves. PMID: 28234971
  4. restoration of miR-218 inhibited retinal angiogenesis via targeting Robo1. MiR-218 contributed to the inhibition of retinal angiogenesis and miR-218 might be a new therapeutic target for preventing retinal neovascularization.. PMID: 26960375
  5. Robo1-Slit2 interaction required for pathfinding mechanism essential to establish the functionally important habenulo-interpeduncular connection. PMID: 25366972
  6. FLRT3 is a Robo1 coreceptor in developing axons. PMID: 24560577
  7. Slit2/Robo1 signaling promotes intestinal tumorigenesis through Src-mediated activation of the Wnt/beta-catenin pathway. PMID: 25605242
  8. Robo1/2 regulate follicle atresia through manipulating granulosa cell apoptosis in mice PMID: 25988316
  9. Roundabout receptor (Robo) genes are expressed in pulmonary neuroendocrine cells (PNECs), a rare, innervated epithelial population. Robo inactivation in mouse lung results in an inability of PNECs to cluster into sensory organoids and triggers increased neuropeptide production upon exposure to air. PMID: 26743624
  10. Cardiac defects in mutants for Robo or Slit range from membranous ventricular septum defects to bicuspid aortic valves. PMID: 25691540
  11. Slit2 signaling through Robo1 and Robo2 has a role in retinal neovascularization PMID: 25894826
  12. This study demonistrated that significant increase of interneurons in the cortices of Robo1-/- mice, implicate Robo1 in the regulation of progenitor cell dynamics in the developing forebrain. PMID: 24741061
  13. we report that Robo1 plays an important role in guiding olfactory sensory neurons in the mouse olfactory system during development. PMID: 23821580
  14. SDF-1-CXCR4 differentially regulates autoimmune diabetogenic T cell adhesion through ROBO1-SLIT2 interactions in mice. PMID: 23811810
  15. These results demonstrate that Robo receptors play a crucial role in neocortical lamination and particularly in the positioning of layers II/III pyramidal neurons. PMID: 22661412
  16. During stroke recovery, a transient reduction in Robo1 expression on the cerebral endothelial cells allowed the uncontrolled infiltration of polymorphonuclear neutrophils into the brain causing inflammatory reactions PMID: 23473743
  17. Prostaglandin F2alpha upregulates Slit2 and Robo1 expression in mouse corpus luteum during luteolysis. PMID: 23814012
  18. Slit/Robo signaling imposes a restriction force on spiral ganglia neurons to ensure their precise positioning for correct spiral ganglia-cochlear hair cells innervations. PMID: 23884932
  19. Slit/Robo1 signaling is involved in regulating neural tube development by tightly coordinating cell proliferation and differentiation during neurulation. PMID: 23438940
  20. Report role of Robo1 in development of the caval veins and pericardium. PMID: 23255421
  21. Inactivation of Robo 1 leads to mispositioning of the stomach in the thoracic instead of the abdominal cavity, which likely contributes to poor lung inflation and lethality at birth, reminiscent of congenital diaphragmatic hernia. PMID: 23328398
  22. This study report that central nervous system progenitors express Robo1 and Robo2, receptors for Slit proteins that regulate axon guidance, and that absence of these receptors or their ligands leads to loss of ventricular mitoses. PMID: 23083737
  23. Modifications to spontaneous calcium activity encode a switch in the axon outgrowth program that allows establishment of specific neuronal connections through the transcriptional regulation of Slit1 and Robo1 signaling. PMID: 22772332
  24. Robo1 and Robo2 are expressed in the nucleus origin of the tract of the postoptic commissure TPOC (nTPOC), while Slit expression domains flank the TPOC trajectory. PMID: 21688288
  25. Robo1 is the predominant receptor for guiding axons in ventral tracts and prevents midline crossing. PMID: 21820427
  26. Axons in the enlarged ventral funiculus of the srGAP3 KO are Robo1 positive but do not express Robo2, indicating that the thickening of the ventral funiculus in the srGAP3 KO is not a Robo2 mediated effect. PMID: 21655271
  27. These results indicate that Slit1/2 - Robo1/2 signaling is critical during the initial establishment of dopaminergic pathways, with roles in the dorsoventral positioning and precise pathfinding of these ascending longitudinal axons. PMID: 21118670
  28. During blood vessel formation microRNA-218 inhibits the expression of Robo1 (and that of Robo2) and thus the heparan sulfate biosynthetic pathway. PMID: 20947829
  29. Robo1 prevents axonal stalling after crossing the floor plate at the spinal cord ventral midline PMID: 20631173
  30. Robo1 inhibits choroidal and retinal angiogenesis in vitro. Robo1 is a potential target for the treatment of choroidal or retinal angiogenesis. PMID: 19958120
  31. Dutt1/Robo1 expression was widespread and diffuse in the lung at embryonic day 17.5 but became increasingly localised to the bronchial epithelium in newborn and adult mice. PMID: 12123796
  32. activation of the transmembrane receptor Activation of Roundabout (Robo) by its ligand, the secreted repulsive guidance cue Slit, inactivates N-cadherin-mediated cell adhesion in CNS growth cones. PMID: 12360290
  33. Data suggest that the Slit family of axon guidance molecules (Slit 1-3) and their Robo 1 and 2 receptors contribute to the topographic targeting of basal vomeronasal axons. PMID: 12954717
  34. In the spinal cord, midline-crossing axons are initially Robo-positive. Midline Robo expression later disappears, but is strongly upregulated in longitudinally running postcrossing axons. PMID: 14689480
  35. Robo1 single mutants show guidance defects that reveal a role for this receptor in guiding commissural axons to different positions within the ventral and lateral funiculi. PMID: 15091338
  36. Slit-2 and Robo-1 expression is present throughout mesenchyme at midgestation and is not detectable by newborn day 1 PMID: 15162513
  37. Dutt1/Robo1 is a classic tumor suppressor gene requiring inactivation of both alleles to elicit lung tumorigenesis in these mice. PMID: 15374951
  38. Robo1 mutants have distinct phenotypes, some of which are different from those described in Slit mutants PMID: 16690755
  39. Robo1 protein directly mediates the repulsive activity of Slit receptors on lateral olfactory tract axons, and is required for normal guidance of these axons in vivo. PMID: 17360927
  40. Our results demonstrate that Robo1 and Robo2 mostly cooperate to mediate the function of Slit proteins in guiding the major forebrain projections. PMID: 17392456
  41. This study shows distinct expression patterns for the Dutt1 and Robo1 alternative promoters in the embryonic nervous system. PMID: 17826360
  42. The role of Slit-Robo1 signaling in the generation, migration and morphological differentiation of cortical interneurons is reported. PMID: 18054781
  43. Robo1 and Robo2 cooperate to prevent premature midline crossing points in the visual pathway of developing brain; Robo1 plays a minor role in retinal ganglion cell targeting. PMID: 18272390
  44. Robo1 and Robo2 cooperate with Slit1 and Slit2 to control the convergence ofolfactory receptor neuron (ORN) axons to the olfactory bulb and the precise targeting of ORN axons to specific glomeruli. PMID: 18417704
  45. Robo1 and Robo2 were largely genetically redundant, and neither appeared to specify specific tract positions. However, combined Robo1 and Robo2 mutations strongly disrupted each pioneer tract. PMID: 18842816
  46. Data show that cerebellofugal axons tend to stall within the midline region in Robo1/2 double knockout mice. PMID: 18986510

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Subcellular Location Cell membrane, Single-pass type I membrane protein, Cell projection, axon
Protein Families Immunoglobulin superfamily, ROBO family
Tissue Specificity Detected in embryonic thalamus neurons (at protein level) (PubMed:24560577). Expressed in embryonal spinal chord. Expressed in embryonal lung, and in adult lung bronchial epithelial cells of large proximal airways.
Database Links

KEGG: mmu:19876

STRING: 10090.ENSMUSP00000023600

UniGene: Mm.310772

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